%0 Journal Article
%A Weierstall, Uwe
%A James, Daniel
%A Wang, Chong
%A White, Thomas
%A Wang, Dingjie
%A Liu, Wei
%A Spence, John C. H.
%A Bruce Doak, R.
%A Nelson, Garrett
%A Fromme, Petra
%A Fromme, Raimund
%A Grotjohann, Ingo
%A Kupitz, Christopher
%A Zatsepin, Nadia A.
%A Liu, Haiguang
%A Basu, Shibom
%A Wacker, Daniel
%A Won Han, Gye
%A Katritch, Vsevolod
%A Boutet, Sébastien
%A Messerschmidt, Marc
%A Williams, Garth J.
%A Koglin, Jason E.
%A Marvin Seibert, M.
%A Klinker, Markus
%A Gati, Cornelius
%A Shoeman, Robert L.
%A Barty, Anton
%A Chapman, Henry N.
%A Kirian, Richard
%A Beyerlein, Kenneth
%A Stevens, Raymond C.
%A Li, Dianfan
%A Shah, Syed T. A.
%A Howe, Nicole
%A Caffrey, Martin
%A Cherezov, Vadim
%T Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
%J Nature Communications
%V 5
%@ 2041-1723
%C London
%I Macmillan Publishers Limited
%M DESY-2014-02800
%P 3309
%D 2014
%X Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000332668300002
%$ pmid:24525480
%R 10.1038/ncomms4309
%U https://bib-pubdb1.desy.de/record/169117