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@ARTICLE{Weierstall:169117,
author = {Weierstall, Uwe and James, Daniel and Wang, Chong and
White, Thomas and Wang, Dingjie and Liu, Wei and Spence,
John C. H. and Bruce Doak, R. and Nelson, Garrett and
Fromme, Petra and Fromme, Raimund and Grotjohann, Ingo and
Kupitz, Christopher and Zatsepin, Nadia A. and Liu, Haiguang
and Basu, Shibom and Wacker, Daniel and Won Han, Gye and
Katritch, Vsevolod and Boutet, Sébastien and Messerschmidt,
Marc and Williams, Garth J. and Koglin, Jason E. and Marvin
Seibert, M. and Klinker, Markus and Gati, Cornelius and
Shoeman, Robert L. and Barty, Anton and Chapman, Henry N.
and Kirian, Richard and Beyerlein, Kenneth and Stevens,
Raymond C. and Li, Dianfan and Shah, Syed T. A. and Howe,
Nicole and Caffrey, Martin and Cherezov, Vadim},
title = {{L}ipidic cubic phase injector facilitates membrane protein
serial femtosecond crystallography},
journal = {Nature Communications},
volume = {5},
issn = {2041-1723},
address = {London},
publisher = {Macmillan Publishers Limited},
reportid = {DESY-2014-02800},
pages = {3309},
year = {2014},
abstract = {Lipidic cubic phase (LCP) crystallization has proven
successful for high-resolution structure determination of
challenging membrane proteins. Here we present a technique
for extruding gel-like LCP with embedded membrane protein
microcrystals, providing a continuously renewed source of
material for serial femtosecond crystallography. Data
collected from sub-10-μm-sized crystals produced with less
than 0.5 mg of purified protein yield structural insights
regarding cyclopamine binding to the Smoothened receptor.},
cin = {FS-CFEL-1},
ddc = {500},
cid = {I:(DE-H253)FS-CFEL-1-20120731},
pnm = {Experiments at CFEL (POF2-544)},
pid = {G:(DE-H253)POF2-CFEL-Exp.-20130405},
experiment = {EXP:(DE-H253)CFEL-Exp-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000332668300002},
pubmed = {pmid:24525480},
doi = {10.1038/ncomms4309},
url = {https://bib-pubdb1.desy.de/record/169117},
}