guest ::
| Home > Publications database > Serial crystallography on in vivo grown microcrystals using synchrotron radiation |
| Home > Publications database > Serial crystallography on in vivo grown microcrystals using synchrotron radiation |
| Journal Article | DESY-2014-01387 |
; ; ; ; ; ; ; ; ; ; ; ; ;
2014
International Union of Crystallography (IUCr)
Chester
This record in other databases: 
Please use a persistent id in citations: doi:10.1107/S2052252513033939
Abstract: Crystal structure determinations of biological macromolecules are limited by the availability of sufficiently sized crystals and by the fact that crystal quality deteriorates during data collection owing to radiation damage. Exploiting a micrometre-sized X-ray beam, high-precision diffractometry and shutterless data acquisition with a pixel-array detector, a strategy for collecting data from many micrometre-sized crystals presented to an X-ray beam in a vitrified suspension is demonstrated. By combining diffraction data from 80 Trypanosoma brucei procathepsin B crystals with an average volume of 9 µm3, a complete data set to 3.0 Å resolution has been assembled. The data allowed the refinement of a structural model that is consistent with that previously obtained using free-electron laser radiation, providing mutual validation. Further improvements of the serial synchrotron crystallography technique and its combination with serial femtosecond crystallography are discussed that may allow the determination of high-resolution structures of micrometre-sized crystals.
; Peer reviewed article
|
The record appears in these collections: |
PDF
