Structure and Conformational Dynamics of DMPC/Dicationic  Surfactant and DMPC/Dicationic Surfactant/DNA Systems

Pietralik, Z.; Kida, W.; Kozak, M.; Andrzejewska, W.; Krzyszton, R.

doi:10.3390/ijms14047642

Journal Article

Structure and Conformational Dynamics of DMPC/Dicationic Surfactant and DMPC/Dicationic Surfactant/DNA Systems

Pietralik, Z. (Corresponding author)Extern* ; Krzyszton, R. (Corresponding author) ; Kida, W. (Corresponding author)Extern* ; Andrzejewska, W. (Corresponding author)Extern* ; Kozak, M.Extern* ; DESY

2013
Molecular Diversity Preservation International Basel

International journal of molecular sciences 14, 7642-7659 (2013) [10.3390/ijms14047642]

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Please use a persistent id in citations: doi:10.3390/ijms14047642

Abstract: Amphiphilic dicationic surfactants, known as gemini surfactants, are currently studied for gene delivery purposes. The gemini surfactant molecule is composed of two hydrophilic "head" groups attached to hydrophobic chains and connected via molecular linker between them. The influence of different concentrations of 1,5-bis (1-imidazolilo-3-decyloxymethyl) pentane chloride (gemini surfactant) on the thermotropic phase behaviour of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bilayers with and without the presence of DNA was investigated using Fourier transformed infrared (FTIR) and circular dichroism (CD) spectroscopies, small angle scattering of synchrotron radiation and differential scanning calorimetry. With increasing concentration of surfactant in DMPC/DNA systems, a disappearance of pretransition and a decrease in the main phase transition enthalpy and temperature were observed. The increasing intensity of diffraction peaks as a function of surfactant concentration also clearly shows the ability of the surfactant to promote the organisation of lipid bilayers in the multilayer lamellar phase.

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