Lipidic phase membrane protein serial femtosecond crystallography

Johansson, L. C.; Shoeman, R. L.; Coppola, N.; Hartmann, R.; Bostedt, C.; Epp, S. W.; Weidenspointner, G.; Ekeberg, T.; Davidsson, J.; Caleman, C.; Hajdu, J.; Nass, K.; Timneanu, N.; Kassemeyer, S.; Soltau, H.; Fromme, P.; Barty, A.; Holl, P.; Hirsemann, H.; Wang, X.; Erk, B.; Seibert, M. M.; DePonte, D. P.; Coffee, R.; Barthelmess, M.; Graafsma, H.; Katona, G.; Rolles, D.; Kirian, R. A.; Hartmann, A.; Chapman, H. N.; Reich, C.; Fleckenstein, H.; Ullrich, J.; Marchesini, S.; Wunderer, C.; Weierstall, U.; Aquila, A.; Kimmel, N.; Stern, S.; Hampton, C. Y.; Spence, J. C. H.; Neutze, R.; Rudenko, A.; Bajt, S.; Gumprecht, L.; Struder, L.; Liang, M.; Stellato, F.; Hunter, M. S.; Foucar, L.; Hauser, G.; Arnlund, D.; Lomb, L.; Martin, A. V.; Rudek, B.; Malmerberg, E.; Andreasson, J.; Sierra, R. G.; Maia, F. R. N. C.; Doak, R. B.; Starodub, D.; Schlichting, I.; White, T. A.; Bozek, J. D.; Schulz, Joachim; Bogan, M. J.; Wahlgren, W. Y.

doi:10.1038/nmeth.1867

Journal Article

Lipidic phase membrane protein serial femtosecond crystallography

Johansson, L. C. ; Arnlund, D.CFEL*DESY* ; White, T. A.CFEL*DESY* ; Katona, G.Extern* ; DePonte, D. P.CFEL*DESY* ; Weierstall, U.Extern* ; Doak, R. B.Extern* ; Shoeman, R. L. ; Lomb, L.Extern* ; Malmerberg, E.CFEL*DESY* ; Davidsson, J. ; Nass, K.CFEL*DESY* ; Liang, M.CFEL*DESY* ; Andreasson, J.Extern* ; Aquila, A.Extern* ; Bajt, S.CFEL*DESY* ; Barthelmess, M.CFEL*DESY* ; Barty, A.CFEL*DESY* ; Bogan, M. J. ; Bostedt, C.Extern* ; Bozek, J. D. ; Caleman, C.CFEL*DESY* ; Coffee, R. ; Coppola, N.XFEL.EU* ; Ekeberg, T.CFEL*DESY* ; Epp, S. W. ; Erk, B.DESY* ; Fleckenstein, H.CFEL*DESY* ; Foucar, L.MPG* ; Graafsma, H.CFEL*DESY* ; Gumprecht, L.CFEL*DESY* ; Hajdu, J.Extern* ; Hampton, C. Y. ; Hartmann, R.Extern* ; Hartmann, A. ; Hauser, G.Extern* ; Hirsemann, H.CFEL*DESY* ; Holl, P. ; Hunter, M. S. ; Kassemeyer, S.Extern* ; Kimmel, N.Extern* ; Kirian, R. A. ; Maia, F. R. N. C. ; Marchesini, S.Extern* ; Martin, A. V.CFEL*DESY* ; Reich, C.Extern* ; Rolles, D.Extern* ; Rudek, B.Extern* ; Rudenko, A.MPG* ; Schlichting, I.Extern* ; Schulz, J.XFEL.EU* ; Seibert, M. M. ; Sierra, R. G. ; Soltau, H. ; Starodub, D.Extern* ; Stellato, F.CFEL*DESY* ; Stern, S.CFEL*DESY* ; Struder, L. ; Timneanu, N.Extern* ; Ullrich, J.MPG* ; Wahlgren, W. Y. ; Wang, X.Extern* ; Weidenspointner, G.XFEL.EU* ; Wunderer, C.CFEL*DESY* ; Fromme, P.Extern* ; Chapman, H. N.CFEL*DESY* ; Spence, J. C. H. ; Neutze, R. ; DESY

2012
Nature Publishing Group London [u.a.] Nature Publishing Group

Nature methods 9, 263-265 (2012) [10.1038/nmeth.1867]

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Please use a persistent id in citations: doi:10.1038/nmeth.1867

Abstract: X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Keyword(s): Crystallography, X-Ray: methods (MeSH) ; Lipid Bilayers: chemistry (MeSH) ; Membrane Proteins: chemistry (MeSH) ; Membrane Proteins: ultrastructure (MeSH) ; Protein Binding (MeSH) ; Protein Conformation: radiation effects (MeSH) ; X-Rays (MeSH) ; Lipid Bilayers ; Membrane Proteins