Journal Article

PUBDB-2025-04821

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png New Generation Stopped-Flow Time-Resolved BioSAXS Experiments at the P12 Beamline

Sutinen, A. (Corresponding author)Extern*EMBL* ; Szpotkowski, K. ; Soloviov, D. ; Trampari, S. ; Donath, T. ; Nikolova, M. ; Bourenkov, G. ; Blanchet, C.

2025
IOP Publ. Bristol

This record in other databases:    

Please use a persistent id in citations: doi:10.1088/1742-6596/3010/1/012155  doi:10.3204/PUBDB-2025-04821

Abstract: Time-resolved small-angle X-ray scattering enables studying dynamic structural changes in biological macromolecules. Developments in fast mixing devices, such as the significant reduction in sample volume requirements, now allow the technique to be applied to a wider range of biologically relevant macromolecules, often available in limited quantities. Advances in synchrotron radiation sources and detector technology have dramatically increased the time resolution, allowing real-time monitoring of reaction kinetics and folding processes at millisecond and sub-millisecond timescales. Here, we present recent updates to the P12 SAXS beamline at EMBL Hamburg, including integrating the new Micro Stopped-Flow Mixer (µSFM) generation. We demonstrated the kinetics of pH-induced conformational dynamics in two case studies of oxygen-carrying proteins, capturing rapid reorganization of the multimeric state on a millisecond time scale. Based on the experimental findings, the new system significantly improves time resolution and sample consumption, facilitating more efficient TR-SAXS experiments.

---

Contributing Institute(s):

1. EMBL-User (EMBL-User)
2. EMBL (EMBL)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)

Experiment(s):

1. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2025

---

Database coverage:
; ; ; Nationallizenz

---