Journal Article

PUBDB-2025-04613

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nanoelectrospray ionization coupled to a linear charge detection array ion trap spectrometer for single viral particle analysis

Maclot, S.Extern* ; Reinert, T. ; Duplantier, L. ; Montagne, G. ; Clavier, C. ; Dagany, X. ; Comby-Zerbino, C. ; Kerleroux, M. ; Antoine, R. (Corresponding author)Extern* ; Thiede, L.Extern*CSSB*DESY* ; Pogan, R.CSSB*XFEL.EU*DESY* ; Uetrecht, C.CSSB*XFEL.EU*DESY* ; Kozhinov, A. N. ; Nagornov, K. O. ; Tsybin, Y. O. ; Papanastasiou, D. (Last author)

2025
AIP Publishing [Melville, NY]

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Please use a persistent id in citations: doi:10.1063/5.0283803  doi:10.3204/PUBDB-2025-04613

Abstract: This work presents the implementation of a new charge detection mass spectrometer (CDMS) design that operates in stand-alone mode, enabled by integration with nanoelectrospray ionization. More specifically, this innovative CDMS consists of a linear charge detection array ion trap spectrometer that combines an eight-tube detector array with conical electrodes. This configuration allows recording data in both transmission mode (linear array) and ion trapping mode (ConeArrayTrap) and measuring the time-of-flight (related to the mass-to-charge ratio) along with the charge of individual ions. Thus, this design supports the high-throughput metrology of viruses at the single-particle level. The devices and geometry of the instrument have been developed based on ion optics simulations. The performance of the current instrument is demonstrated using human norovirus-like particles (hNoVLP) and Adenovirus Ad(5) (hAdV5).

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Contributing Institute(s):

1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) / DESY - Charlotte Uetrecht (CSSB-LIV/DESY-CU)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. ARIADNE - Redefining mass spectrometry – a breakthrough platform for real-time noninvasive breath analysis with single ion detection of intact viruses and bacteria and post-analysis molecular characterization (964553) (964553)
3. VIRUSong - Vibrations of viral particles scatter light (101099058) (101099058)
4. VIRUSCAN - Optomechanics for Virology (731868) (731868)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2025

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Database coverage:
; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; National-Konsortium ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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