Journal Article

PUBDB-2025-04167

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Design and implementation of aerobic and ambient CO2-reduction as an entry-point for enhanced carbon fixation

Satanowski, A. (Corresponding author) ; Marchal, D. G. ; Perret, A. ; Petit, J.-L. ; Bouzon, M. ; Döring, V. ; Dubois, I. ; He, H. ; Smith, E. N. ; Pellouin, V. ; Petri, H. M. ; Rainaldi, V. ; Nattermann, M. ; Burgener, S. ; Paczia, N. ; Zarzycki, J. ; Heinemann, M. ; Bar-Even, A. ; Erb, T. (Corresponding author)Extern*XFEL.EU*

2025
Springer Nature [London]

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Please use a persistent id in citations: doi:10.1038/s41467-025-57549-4  doi:10.3204/PUBDB-2025-04167

Abstract: The direct reduction of CO2 into one-carbon molecules is key to highly efficient biological CO2-fixation. However, this strategy is currently restricted to anaerobic organisms and low redox potentials. In this study, we introduce the CORE cycle, a synthetic metabolic pathway that converts CO2 to formate at aerobic conditions and ambient CO2 levels, using only NADPH as a reductant. Combining theoretical pathway design and analysis, enzyme bioprospecting and high-throughput screening, modular assembly and adaptive laboratory evolution, we realize the CORE cycle in vivo and demonstrate that the cycle supports growth of E. coli by supplementing C1-metabolism and serine biosynthesis from CO2. We further analyze the theoretical potential of the CORE cycle as a new entry-point for carbon in photorespiration and autotrophy. Overall, our work expands the solution space for biological carbon reduction, offering a promising approach to enhance CO2 fixation processes such as photosynthesis, and opening avenues for synthetic autotrophy.

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Contributing Institute(s):

1. EMBL-User (EMBL-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. GAIN4CROPS - Rewiring photorespiration using natural and synthetic pathways to sustainably increase crop yield (862087) (862087)

Experiment(s):

1. PETRA Beamline P13 (PETRA III)
2. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2025

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