Journal Article

PUBDB-2025-02618

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography

Schulz, E. (Corresponding author)Extern* ; Prester, A.Extern*EMBL* ; Stetten, D. v.Extern* ; Gore, G.Extern* ; Hatton, C.Extern* ; Bartels, K.CSSB*EMBL* ; Leimkohl, J.-P.CFEL*MPG* ; Schikora, H.CFEL*MPG* ; Ginn, H.CFEL*XFEL.EU*DESY* ; Tellkamp, F. (Corresponding author)CFEL*MPG* ; Mehrabi, P. (Corresponding author)Extern*MPG*

2025
Springer Nature [London]

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Please use a persistent id in citations: doi:10.1038/s41467-025-61631-2  doi:10.3204/PUBDB-2025-02618

Abstract: The vast majority of protein structures are determined at cryogenic temperatures, which are far from physiological conditions. Nevertheless, it is well established that temperature is an essential thermodynamic parameter for understanding the conformational dynamics and functionality of proteins in their native environments. Time-resolved crystallography is a technique that aims to elucidate protein function by examining structural alterations during processes such as ligand binding, catalysis, or allostery. However, this approach is typically conducted under ambient conditions, which may obscure crucial conformational states, that are only visible at physiological temperatures. In this study, we directly address the interplay between protein structure and activity via a method that enables multi-temperature, time-resolved serial crystallography experiments in a temperature window from below 10 °C to above 70 °C. Via this 5D-SSX, time-resolved experiments can now be carried out at physiological temperatures and with long time delays, providing insights into protein function and enzyme catalysis. Our findings demonstrate the temperature-dependent modulation of turnover kinetics for the mesophilic β-lactamase CTX-M-14 and the thermophilic enzyme xylose isomerase, within the full protein structure.

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Contributing Institute(s):

1. Forschungsgruppe für strukturelle Dynamik (MPSD)
2. EMBL-User (EMBL-User)
3. PETRA III (PETRA III)
4. Fachgruppe DNMX (FS-CFEL-1-DNMX)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. DFG project G:(GEPRIS)194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
4. DFG project G:(GEPRIS)451079909 - Untersuchung allosterischer Mechanismen durch zeitaufgelöste serielle Synchrotronkristallographie (451079909) (451079909)
5. DFG project G:(GEPRIS)458246365 - Zeitaufgelöste Strukturanalysde der extended spectrum Beta-Lactamase CTX-M-14 (458246365) (458246365)
6. DynaPLIX - Dynamics of Protein–Ligand Interactions (101071843) (101071843)

Experiment(s):

1. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2025

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