Journal Article

PUBDB-2025-02328

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png CryoRhodopsins: A comprehensive characterization of a group of microbial rhodopsins from cold environments

Lamm, G. H. U. ; Marin, E. ; Alekseev, A. ; Schellbach, A. V. ; Stetsenko, A. ; Haro-Moreno, J. M. ; Bourenkov, G.XFEL.EU*EMBL* ; Borshchevskiy, V. ; Asido, M. ; Agthe, M.Extern*EMBL* ; Engilberge, S. ; Rose, S. L. ; Caramello, N. ; Royant, A. ; Schneider, T. R.EMBL* ; Bateman, A. ; Mager, T. ; Moser, T. ; Rodriguez-Valera, F. ; Wachtveitl, J. (Corresponding author) ; Guskov, A. (Corresponding author)Extern*EMBL* ; Kovalev, K. (Corresponding author)Extern*EMBL*

2025
Assoc. Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/sciadv.adv1015  doi:10.3204/PUBDB-2025-02328

Abstract: Microbial rhodopsins are omnipresent on Earth; however, the vast majority of them remain uncharacterized. Here, we describe a rhodopsin group found in microorganisms from cold environments, such as glaciers, denoted as CryoRhodopsins (CryoRs). A distinguishing feature of the group is the presence of a buried arginine residue close to the cytoplasmic face. Combining single-particle cryo–electron microscopy and x-ray crystallography with rhodopsin activation by light, we demonstrate that the arginine stabilizes an ultraviolet (UV)–absorbing intermediate of an extremely slow CryoRhodopsin photocycle. Together with extensive spectroscopic characterization, our investigations on CryoR1 and CryoR2 proteins reveal mechanisms of photoswitching in the identified group. Our data suggest that CryoRs are sensors for UV irradiation and are also capable of inward proton translocation modulated by UV light.

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Contributing Institute(s):

1. EMBL-User (EMBL-User)
2. EMBL (EMBL)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. EIPOD4 - EMBL Interdisciplinary Postdoc Programme 4 (847543) (847543)
3. DFG project G:(GEPRIS)390729940 - EXC 2067: Multiscale Bioimaging: Von molekularen Maschinen zu Netzwerken erregbarer Zellen (390729940) (390729940)

Experiment(s):

1. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2025

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