Journal Article

PUBDB-2024-06630

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png ATP-Triggered Fe(CN)$_2$CO Synthon Transfer from the Maturase HypCD to the Active Site of Apo-[NiFe]-Hydrogenase

Kwiatkowski, A. (First author) ; Caserta, G. (Corresponding author) ; Schulz, A.-C. ; Frielingsdorf, S. ; Pelmenschikov, V. ; Weisser, K. ; Belsom, A. ; Rappsilber, J. ; Sergueev, I.XFEL.EU*DESY* ; Limberg, C. ; Mroginski, M.-A. ; Zebger, I. ; Lenz, O. (Corresponding author)

2024
ACS Publications Washington, DC

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Please use a persistent id in citations: doi:10.1021/jacs.4c09791  doi:10.3204/PUBDB-2024-06630

Abstract: [NiFe]-hydrogenases catalyze the reversible activation of H$_2$ using a unique NiFe(CN)2CO metal site, which is assembled by a sophisticated multiprotein machinery. The [4Fe–4S] cluster-containing HypCD complex, which possesses an ATPase activity with a hitherto unknown function, serves as the hub for the assembly of the Fe(CN)$_2$CO subfragment. HypCD is also thought to be responsible for the subsequent transfer of the iron fragment to the apo-form of the catalytic hydrogenase subunit, but the underlying mechanism has remained unexplored. Here, we performed a thorough spectroscopic characterization of different HypCD preparations using infrared, Mössbauer, and NRVS spectroscopy, revealing molecular details of the coordination of the Fe(CN)$_2$CO fragment. Moreover, biochemical assays in combination with spectroscopy, AlphaFold structure predictions, protein–ligand docking calculations, and crosslinking MS deciphered unexpected mechanistic aspects of the ATP requirement of HypCD, which we found to actually trigger the transfer of the Fe(CN)$_2$CO fragment to the apo-hydrogenase.

Note: Additional Funding: COSTAction FeSImmChemNet, CA21115, supported by COST(European Cooperation in Science and Technology). The Wellcome Centre for Cell Biology is supported by core fundingfrom the Wellcome Trust [203149] (J.R.).

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)
2. Experimentebetreuung PETRA III (FS-PET-S)

Research Program(s):

1. 631 - Matter – Dynamics, Mechanisms and Control (POF4-631) (POF4-631)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. FS-Proposal: I-20220851 (I-20220851) (I-20220851)
4. FS-Proposal: I-20210325 (I-20210325) (I-20210325)
5. DFG project G:(GEPRIS)311062227 - Die Rolle von Eisen-Schwefel-Kofaktoren in der Assemblierung von Metallzentren und der Katalyse von Hydrogenasen (311062227) (311062227)
6. DFG project G:(GEPRIS)449713269 - Design, Synthese und Analyse von photospaltbaren chemischen Crosslinkern zur Entwicklung von verbesserten Methoden in der Crosslinking Massenspektrometrie (449713269) (449713269)
7. DFG project G:(GEPRIS)390540038 - EXC 2008: Unifying Systems in Catalysis "UniSysCat" (390540038) (390540038)

Experiment(s):

1. PETRA Beamline P01 (PETRA III)

Appears in the scientific report 2024

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