Journal Article PUBDB-2024-06074

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png
Assembly of Differently Sized Supercharged Protein Nanocages into Superlattices for Construction of Binary Nanoparticle–Protein Materials

 ;  ;  ;  ;  ;  ;  ;  ;  ;  ;

2024
Soc. Washington, DC

ACS nano 18(36), 25325-25336 () [10.1021/acsnano.4c09551]
 GO

This record in other databases:        

Please use a persistent id in citations: doi:  doi:

Abstract: This study focuses on the design and characterization of binary nanoparticle superlattices: Two differently sized, supercharged protein nanocages are used to create a matrix for nanoparticle arrangement. We have previously established the assembly of protein nanocages of the same size. Here, we present another approach for multicomponent biohybrid material synthesis by successfully assembling two differently sized supercharged protein nanocages with different symmetries. Typically, the ordered assembly of objects with nonmatching symmetry is challenging, but our electrostatic-based approach overcomes the symmetry mismatch by exploiting electrostatic interactions between oppositely charged cages. Moreover, our study showcases the use of nanoparticles as a contrast enhancer in an elegant way to gain insights into the structural details of crystalline biohybrid materials. The assembled materials were characterized with various methods, including transmission electron microscopy (TEM) and single-crystal small-angle X-ray diffraction (SC-SAXD). We employed cryo-plasma-focused ion beam milling (cryo-PFIB) to prepare lamellae for the investigation of nanoparticle sublattices via electron cryo-tomography. Importantly, we refined superlattice structure data obtained from single-crystal SAXD experiments, providing conclusive evidence of the final assembly type. Our findings highlight the versatility of protein nanocages for creating distinctive types of binary superlattices. Because the nanoparticles do not influence the type of assembly, protein cage matrices can combine various nanoparticles in the solid state. This study not only contributes to the expanding repertoire of nanoparticle assembly methods but also demonstrates the power of advanced characterization techniques in elucidating the structural intricacies of these biohybrid materials.

Classification:

Contributing Institute(s):
  1. DOOR-User (DOOR ; HAS-User)
  2. Nanolab (FS-NL)
  3. CSSB-CF-CRYO (CSSB-CF-CRYO)
  4. Fachgruppe DNMX (FS-CFEL-1-DNMX)
Research Program(s):
  1. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)
  2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
  3. DFG project G:(GEPRIS)390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
  4. FS-Proposal: I-20211171 (I-20211171) (I-20211171)
Experiment(s):
  1. PETRA Beamline P11 (PETRA III)
  2. PETRA Beamline P14 (PETRA III)
  3. DESY NanoLab: Microscopy
  4. PETRA Beamline P62 (PETRA III)
  5. DESY NanoLab: Sample Preparation

Appears in the scientific report 2024
Database coverage:
Medline ; Creative Commons Attribution CC BY 4.0 ; OpenAccess ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF >= 15 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection
Click to display QR Code for this record

The record appears in these collections:
Private Collections > >CFEL > >FS-CFEL > FS-CFEL-1-DNMX
Private Collections > >Extern > >HAS-User > HAS-User
Private Collections > >DESY > >FS > FS-NL
Private Collections > >CSSB > CSSB-CF-CRYO
Document types > Articles > Journal Article
Public records
Publications database
OpenAccess

 Record created 2024-09-27, last modified 2025-07-15


OpenAccess:
Download fulltext PDF Download fulltext PDF (PDFA)
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)