Journal Article

PUBDB-2024-05686

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Akkermansia muciniphila exoglycosidases target extended blood group antigens to generate ABO-universal blood

Jensen, M. ; Stenfelt, L. ; Ricci Hagman, J. ; Pichler, M. J. ; Weikum, J. ; Nielsen, T. S. ; Hult, A. ; Morth, J. P. ; Olsson, M. L. (Corresponding author) ; Abou Hachem, M. (Corresponding author)

2024
Nature Publishing Group London

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Please use a persistent id in citations: doi:10.1038/s41564-024-01663-4

Abstract: Matching donor and recipient blood groups based on red blood cell (RBC) surface ABO glycans and antibodies in plasma is crucial to avoid potentially fatal reactions during transfusions. Enzymatic conversion of RBC glycans to the universal group O is an attractive solution to simplify blood logistics and prevent ABO-mismatched transfusions. The gut symbiont Akkermansia muciniphila can degrade mucin O-glycans including ABO epitopes. Here we biochemically evaluated 23 Akkermansia glycosyl hydrolases and identified exoglycosidase combinations which efficiently transformed both A and B antigens and four of their carbohydrate extensions. Enzymatic removal of canonical and extended ABO antigens on RBCs significantly improved compatibility with group O plasmas, compared to conversion of A or B antigens alone. Finally, structural analyses of two B-converting enzymes identified a previously unknown putative carbohydrate-binding module. This study demonstrates the potential utility of mucin-degrading gut bacteria as valuable sources of enzymes for production of universal blood for transfusions.

Note: We acknowledge DESY for time on the P13 beamline at Petra III under the proposal MX846 and would like to thank I. Bento for assistance in using the beamline and data collection.the proposal MX846 and would like to thank I. Bento for assistance in using the beamline and data collection. Waiting for fulltext

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Contributing Institute(s):

1. EMBL-User (EMBL-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. FS-Proposal: I-20190334 (I-20190334) (I-20190334)
3. FS-Proposal: I-20200120 (I-20200120) (I-20200120)

Experiment(s):

1. PETRA Beamline P13 (PETRA III)

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DEAL Nature ; Essential Science Indicators ; IF >= 25 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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