Journal Article

PUBDB-2024-01056

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png MARVEL domain containing CMTM4 affects CXCR4 trafficking

Bona, A. (Corresponding author) ; Seifert, M. ; Thünauer, R.Extern*CSSB* ; Zodel, K. ; Frew, I. J. ; Römer, W.Extern* ; Walz, G. ; Yakulov, T. A. (Corresponding author)

2022
American Society for Cell Biology Bethesda, Md.

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Please use a persistent id in citations: doi:10.1091/mbc.E22-05-0152

Abstract: Sections View article Tools ShareAbstractThe MARVEL proteins CMTM4 and CMTM6 control PD-L1, thereby influencing tumor immunity. We found that defective zebrafish cmtm4 slowed the development of the posterior lateral line (pLL) by altering the Cxcr4b gradient across the pLL primordium (pLLP). Analysis in mammalian cells uncovered that CMTM4 interacted with CXCR4, altering its glycosylation pattern, but did not affect internalization or degradation of CXCR4 in the absence of its ligand CXCL12. Synchronized release of CXCR4 from the endoplasmic reticulum revealed that CMTM4 slowed CXCR4 trafficking from the endoplasmic reticulum to the plasma membrane without affecting overall cell surface expression. Altered CXCR4 trafficking reduced ligand-induced CXCR4 degradation and affected AKT but not ERK1/2 activation. CMTM4 expression, in contrast to that of CXCR4, correlated with the survival of patients with renal cell cancer in the TCGA cohort. Furthermore, we observed that cmtm4 depletion promotes the separation of cells from the pLLP cell cluster in zebrafish embryos. Collectively, our findings indicate that CMTM4 exerts general roles in the biosynthetic pathway of cell surface molecules and seems to affect CXCR4-dependent cell migration.

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Contributing Institute(s):

1. CSSB-CF-ALFM (CSSB-CF-ALFM)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. DFG project G:(GEPRIS)390939984 - EXC 2189: CIBSS - Centre for Integrative Biological Signalling Studies (390939984) (390939984)
3. DFG project G:(GEPRIS)39236281 - EXC 294: BIOSS Zentrum für Biologische Signalstudien - von der Analyse zur Synthese (39236281) (39236281)

Experiment(s):

1. No specific instrument

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Essential Science Indicators ; IF < 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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