Home > Publications database > Laser-induced alignment of nanoparticles and macromolecules for single-particle-imaging applications |
Journal Article | PUBDB-2023-03635 |
; ; ;
2025
ACS Publications
Washington, DC
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Please use a persistent id in citations: doi:10.1021/jacs.4c15679 doi:10.3204/PUBDB-2023-03635
Abstract: Laser-induced alignment of particles and molecules was long envisioned to support three-dimensional structure determination using “single-molecule diffraction” with X-ray free-electron lasers [PRL 92, 198102 (2004)]. However, the alignment of isolated macromolecules has not yet been demonstrated also because quantitative modeling is very expensive. We computationally demonstrated that the alignment of nanorods and proteins is possible with a standard laser technology. We performed a comprehensive analysis on the dependence of the degree of alignment on molecular properties and experimental details, e.g., particle temperature and laser-pulse energy. Considering the polarizability anisotropy of about 150,000 proteins, our analysis revealed that most of these proteins can be aligned using realistic experimental parameters.
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