Journal Article

PUBDB-2023-01521

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An Optimized Approach for Serial Crystallography Using Chips

Galchenkova, M.CFEL*XFEL.EU*DESY* ; Rahmani Mashhour, A.CFEL*DESY* ; Reinke, P.CFEL*DESY* ; Meyer, J.Extern* ; Chapman, H. N.CFEL*XFEL.EU*DESY* ; Yefanov, O. (Corresponding author)CFEL*XFEL.EU*DESY*

2023
MDPI Basel

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Please use a persistent id in citations: doi:10.3390/cryst13081225  doi:10.3204/PUBDB-2023-01521

Abstract: Serial crystallography is a rapidly developing field that makes it possible to determine the structure of biomolecules at room temperature with atomic resolution. Numerous advances in detectors, data analysis pipelines, sample delivery methods, and crystallization protocols expand the scope of structural biology to understand the fundamental processes that take place in living cells. At the same time, all stages of experiments should be maximally optimized to avoid loss of beamtime. Thus, this paper proposes a strategy for optimizing beamtime utilization while using a fixed target sample delivery method such as chips. The strategy consists of two steps: first, a fast raster scan of the chip is performed to determine the positions of the crystals, and then small rotational series are measured at predetermined positions. Such an approach skips empty positions during data acquisition, saving valuable beam time and, as an additional consequence, reducing the volume of measured data.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. FS-CFEL-1 Fachgruppe BMX (FS-CFEL-1-BMX)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. InternLabs-0011 - HIR3X - Helmholtz International Laboratory on Reliability, Repetition, Results at the most advanced X-ray Sources (2020_InternLabs-0011) (2020_InternLabs-0011)
4. FISCOV - FISCOV Helmholtz large research infrastructures in the fight against epidemic outbreaks (FISCOV) (FISCOV)

Experiment(s):

1. PETRA Beamline P11 (PETRA III)

Appears in the scientific report 2023

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Database coverage:
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