Journal Article

PUBDB-2023-01370

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Confinement of Triple-Enzyme-Involved Antioxidant Cascade in Two-Dimensional Nanostructure

Szerlauth, A. ; Varga, Á. ; Madácsy, T. ; Sebők, D. ; Bashiri, S. ; Skwarczynski, M. ; Toth, I. ; Maléth, J. (Corresponding author) ; Szilagyi, I. (Corresponding author)

2023
ACS Publications Washington, DC

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Please use a persistent id in citations: doi:10.1021/acsmaterialslett.2c00580  doi:10.3204/PUBDB-2023-01370

Abstract: Application of antioxidant enzymes in medical or industrial processes is limited due to their high sensitivity to environmental conditions. Incorporation of such enzymes in nanostructures provides a promising route to obtain highly efficient and robust biocatalytic system to scavenge reactive oxygen species (ROS). Here, this question was addressed by confinement of superoxide dismutase (SOD), horseradish peroxidase (HRP), and catalase (CAT) enzymes into nanostructures containing polyelectrolyte building blocks (alginate (Alg) and trimethyl chitosan (TMC)) and delaminated layered double hydroxide (dLDH) nanoparticle support. The nanocomposite possessed excellent structural and colloidal stability, while antioxidant tests revealed that the enzymes remained active upon immobilization and the developed composite greatly reduced intracellular oxidative stress in two-dimensional cell cultures. Moreover, it effectively prevented hydrogen peroxide-induced double stranded DNA breaks, which is a common consequence of oxidative stress. The results provide important tools to design complex nanostructures with multienzymatic antioxidant activities for ROS scavenging.

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Contributing Institute(s):

1. EMBL-User (EMBL-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)

Experiment(s):

1. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2023

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF >= 10 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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