Journal Article

PUBDB-2022-07829

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Marine bacteroidetes use a conserved enzymatic cascade to digest diatom β-mannan

Beidler, I. ; Robb, C. S.Extern*EMBL* ; Vidal-Melgosa, S. ; Zühlke, M.-K. ; Bartosik, D. ; Solanki, V.Extern*EMBL* ; Markert, S. ; Becher, D. ; Schweder, T. (Corresponding author) ; Hehemann, J.-H. (Corresponding author)Extern*EMBL*

2022
Nature Publishing Group Basingstoke

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Please use a persistent id in citations: doi:10.1038/s41396-022-01342-4  doi:10.3204/PUBDB-2022-07829

Abstract: The polysaccharide β-mannan, which is common in terrestrial plants but unknown in microalgae, was recently detected during diatom blooms. We identified a β-mannan polysaccharide utilization locus (PUL) in the genome of the marine flavobacterium Muricauda sp. MAR_2010_75. Proteomics showed β-mannan induced translation of 22 proteins encoded within the PUL. Biochemical and structural analyses deduced the enzymatic cascade for β-mannan utilization. A conserved GH26 β-mannanase with endo-activity depolymerized the β-mannan. Consistent with the biochemistry, X-ray crystallography showed the typical TIM-barrel fold of related enzymes found in terrestrial β-mannan degraders. Structural and biochemical analyses of a second GH26 allowed the prediction of an exo-activity on shorter manno-gluco oligosaccharides. Further analysis demonstrated exo-α-1,6-galactosidase- and endo-β-1,4-glucanase activity of the PUL-encoded GH27 and GH5_26, respectively, indicating the target substrate is a galactoglucomannan. Epitope deletion assays with mannanases as analytic tools indicate the presence of β-mannan in the diatoms Coscinodiscus wailesii and Chaetoceros affinis. Mannanases from the PUL were active on diatom β-mannan and polysaccharide extracts sampled during a microalgal bloom at the North Sea. Together these results demonstrate that marine microorganisms use a conserved enzymatic cascade to degrade β-mannans of marine and terrestrial origin and that this metabolic pathway plays a role in marine carbon cycling.

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Contributing Institute(s):

1. Max-Planck-Gesellschaft (MPG)
2. DOOR-User (DOOR ; HAS-User)
3. EMBL-User (EMBL-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)

Experiment(s):

1. PETRA Beamline P11 (PETRA III)

Appears in the scientific report 2022

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; DEAL Springer ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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