Journal Article

PUBDB-2021-04915

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Homogeneous batch micro-crystallization of proteins from ammonium sulfate

Stohrer, C. ; Horrell, S.Extern* ; Meier, S.CFEL*EMBL* ; Sans, M.Extern*EMBL* ; von Stetten, D.EMBL* ; Hough, M. ; Goldman, A. ; Monteiro, D. C. F. (Corresponding author) ; Pearson, A. (Corresponding author)Extern*XFEL.EU*EMBL*

2021
Wiley Bognor Regis

This record in other databases:        

Please use a persistent id in citations: doi:10.1107/S2059798320015454  doi:10.3204/PUBDB-2021-04915

Abstract: The emergence of X-ray free-electron lasers has led to the development of serial macromolecular crystallography techniques, making it possible to study smaller and more challenging crystal systems and to perform time-resolved studies on fast time scales. For most of these studies the desired crystal size is limited to a few micrometres, and the generation of large amounts of nanocrystals or microcrystals of defined size has become a bottleneck for the wider implementation of these techniques. Despite this, methods to reliably generate microcrystals and fine-tune their size have been poorly explored. Working with three different enzymes, L-aspartate $α$-decarboxylase, copper nitrite reductase and copper amine oxidase, the precipitating properties of ammonium sulfate were exploited to quickly transition from known vapour-diffusion conditions to reproducible, large-scale batch crystallization, circumventing the tedious determination of phase diagrams. Furthermore, the specific ammonium sulfate concentration was used to fine-tune the crystal size and size distribution. Ammonium sulfate is a common precipitant in protein crystallography, making these findings applicable to many crystallization systems to facilitate the production of large amounts of microcrystals for serial macromolecular crystallography experiments.

---

Contributing Institute(s):

1. EMBL-User (EMBL-User)
2. EMBL (EMBL)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)

Experiment(s):

1. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2021

---

Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---