Preprint PUBDB-2021-04863

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png
N-terminal phosphorylation regulates the activity of Glycogen Synthase Kinase 3 from Plasmodium falciparum

 ;  ;  ;  ;  ;

2021

This record in other databases:    

Please use a persistent id in citations: doi:  doi:

Abstract: As the decline of malaria cases stalled over the last five years, novel targets in Plasmodium falciparum are necessary for the development of new drugs. Glycogen Synthase Kinase (PfGSK3) has been identified as a potential target, since its selective inhibitors were shown to disrupt the parasite’s life cycle. Here, we show that PfGSK3 exhibits autophosphorylation, leading to an extensive phosphorylation both in vitro and in the parasite. In the uncanonical N-terminal region of the parasite enzyme, we identified several autophosphorylation sites that regulate the activity of PfGSK3. By combining molecular modeling with experimental small-angle X-ray scattering data, we show that increased PfGSK3 activity is promoted by conformational changes in the PfGSK3 N-terminus, triggered by N-terminal phosphorylation. Our work provides novel insights into the structure and regulation of the malarial PfGSK3.


Contributing Institute(s):
  1. EMBL (EMBL)
  2. EMBL-User (EMBL-User)
  3. CSSB-EMBL (CSSB-EMBL)
  4. CSSB-EMBL-CL (CSSB-EMBL-CL)
  5. CSSB-BNITM-TG (CSSB-BNITM-TG)
Research Program(s):
  1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
Experiment(s):
  1. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2021
Database coverage:
Creative Commons Attribution CC BY 4.0 ; OpenAccess
Click to display QR Code for this record

The record appears in these collections:
Private Collections > >CSSB > CSSB-BNITM-TG
Private Collections > >CSSB > CSSB-EMBL-CL
Private Collections > >EMBL > EMBL-User
Private Collections > >EMBL > CSSB-EMBL
Private Collections > >EMBL > EMBL
Document types > Reports > Preprints
Public records
Publications database
OpenAccess

 Record created 2021-11-28, last modified 2025-07-24


OpenAccess:
Download fulltext PDF Download fulltext PDF (PDFA)
External link:
Download fulltextFulltext
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)