Journal Article

PUBDB-2020-05046

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

Li, H. ; Nazari, R. ; Abbey, B. ; Alvarez, R. ; Aquila, A. (Corresponding author)Extern* ; Ayyer, K.CFEL*MPG*XFEL.EU*EMBL* ; Barty, A.CFEL*DESY* ; Berntsen, P. ; Bielecki, J. ; Pietrini, A. ; Bucher, M. ; Carini, G. ; Chapman, H. N.CFEL*DESY* ; Contreras, A. ; Daurer, B. J. ; DeMirci, H. ; Flűckiger, L. ; Frank, M. ; Hajdu, J. ; Hantke, M. F. ; Hogue, B. G. ; Hosseinizadeh, A. ; Hunter, M. S. ; Jönsson, H. O. ; Kirian, R.Extern*XFEL.EU* ; Kurta, R. P. ; Loh, D. ; Maia, F. R. N. C. ; Mancuso, A. P. ; Morgan, A. J. (Corresponding author)CFEL*XFEL.EU* ; McFadden, M. ; Muehlig, K. ; Munke, A.CFEL*XFEL.EU*DESY* ; Reddy, H. K. N. ; Nettelblad, C. ; Ourmazd, A. ; Rose, M. ; Schwander, P. ; Marvin Seibert, M. ; Sellberg, J. A. ; Sierra, R. G. ; Sun, Z. ; Svenda, M. ; Vartanyants, I. A.DESY* ; Walter, P. ; Westphal, D. ; Williams, G. ; Xavier, P. L. ; Yoon, C. H. ; Zaare, S.

2020
Nature Publ. Group London

This record in other databases:        

Please use a persistent id in citations: doi:10.1038/s41597-020-00745-2  doi:10.3204/PUBDB-2020-05046

Abstract: Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

---

Contributing Institute(s):

1. Forschungsgruppe für strukturelle Dynamik (MPSD)
2. Computational Nanoscale Imaging (CFEL-CNI)
3. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
4. FS_FS-SC (FS_FS-SC)
5. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2020

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---