Journal Article

PUBDB-2019-04150

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Molecular adaptations of NADP-malic enzyme for its function in C4 photosynthesis in grasses

Alvarez, C. E. ; Bovdilova, A. ; Höppner, A.Extern*EMBL* ; Wolff, C.-C. ; Saigo, M. ; Trajtenberg, F. ; Zhang, T. ; Buschiazzo, A. ; Nagel-Steger, L. ; Drincovich, M. F. ; Lercher, M. J. ; Maurino, V. G. (Corresponding author)

2019
Nature Publ. Group London

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Please use a persistent id in citations: doi:10.1038/s41477-019-0451-7

Abstract: In C4 grasses of agronomical interest, malate shuttled into the bundle sheath cells is decarboxylated mainly by nicotinamide adenine dinucleotide phosphate (NADP)-malic enzyme (C4-NADP-ME). The activity of C4-NADP-ME was optimized by natural selection to efficiently deliver CO2 to Rubisco. During its evolution from a plastidic non-photosynthetic NADP-ME, C4-NADP-ME acquired increased catalytic efficiency, tetrameric structure and pH-dependent inhibition by its substrate malate. Here, we identified specific amino acids important for these C4 adaptions based on strict differential conservation of amino acids, combined with solving the crystal structures of maize and sorghum C4-NADP-ME. Site-directed mutagenesis and structural analyses show that Q503, L544 and E339 are involved in catalytic efficiency; E339 confers pH-dependent regulation by malate, F140 is critical for the stabilization of the oligomeric structure and the N-terminal region is involved in tetramerization. Together, the identified molecular adaptations form the basis for the efficient catalysis and regulation of one of the central biochemical steps in C4 metabolism.

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Contributing Institute(s):

1. EMBL-User (EMBL-User)

Research Program(s):

1. 6G3 - PETRA III (POF3-622) (POF3-622)
2. DFG project 390686111 - EXC 2048: CEPLAS - Exzellenzcluster für Pflanzenwissenschaften (390686111) (390686111)

Experiment(s):

1. PETRA Beamline P13 (PETRA III)

Appears in the scientific report 2019

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; No Authors Fulltext ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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