Journal Article

PUBDB-2018-05837

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser

Nogly, P.Extern* ; Weinert, T. ; James, D. ; Carbajo, S. ; Ozerov, D.Extern* ; Furrer, A. ; Gashi, D. ; Borin, V. ; Skopintsev, P.Extern* ; Jaeger, K. ; Nass, K. ; Båth, P. ; Bosman, R.Extern* ; Koglin, J. ; Seaberg, M. ; Lane, T. ; Kekilli, D.Extern* ; Brünle, S.Extern* ; Tanaka, T. ; Wu, W. ; Milne, C. ; White, T.CFEL*DESY* ; Barty, A.CFEL* ; Weierstall, U. ; Panneels, V.Extern* ; Nango, E. ; Iwata, S. ; Hunter, M.Extern* ; Schapiro, I. ; Schertler, G. ; Neutze, R. ; Standfuss, J. (Corresponding author)

2018
Moses King Cambridge, Mass.

This record in other databases:        

Please use a persistent id in citations: doi:10.1126/science.aat0094  doi:10.3204/PUBDB-2018-05837

Abstract: Ultrafast isomerization of retinal is the primary step in photoresponsive biological functions including vision in humans and ion transport across bacterial membranes. We used an x-ray laser to study the subpicosecond structural dynamics of retinal isomerization in the light-driven proton pump bacteriorhodopsin. A series of structural snapshots with near-atomic spatial resolution and temporal resolution in the femtosecond regime show how the excited all-trans retinal samples conformational states within the protein binding pocket before passing through a twisted geometry and emerging in the 13-cis conformation. Our findings suggest ultrafast collective motions of aspartic acid residues and functional water molecules in the proximity of the retinal Schiff base as a key facet of this stereoselective and efficient photochemical reaction.

Note: © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science

---

Contributing Institute(s):

1. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
2. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2018

---

Database coverage:
; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; IF >= 40 ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; Nationallizenz ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---