Journal Article

PUBDB-2016-02690

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Room-Temperature Macromolecular Crystallography Using a Micro-Patterned Silicon Chip with Minimal Background Scattering

Roedig, P. (Corresponding author)DESY* ; Duman, R. ; Sanchez-Weatherby, J. ; Vartiainen, I. ; Burkhardt, A.DESY* ; Warmer, M.DESY* ; David, C. ; Wagner, A. ; Meents, A.DESY*

2016
Munksgaard Copenhagen

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Please use a persistent id in citations: doi:10.1107/S1600576716006348  doi:10.3204/PUBDB-2016-02690

Abstract: Recent success at X-ray free-electron lasers has led to serial crystallography experiments staging a comeback at synchrotron sources as well. With crystal lifetimes typically in the millisecond range and the latest-generation detector technologies with high framing rates up to 1 kHz, fast sample exchange has become the bottleneck for such experiments. A micro-patterned chip has been developed from single-crystalline silicon, which acts as a sample holder for up to several thousand microcrystals at a very low background level. The crystals can be easily loaded onto the chip and excess mother liquor can be efficiently removed. Dehydration of the crystals is prevented by keeping them in a stream of humidified air during data collection. Further sealing of the sample holder, for example with Kapton, is not required. Room-temperature data collection from insulin crystals loaded onto the chip proves the applicability of the chip for macromolecular crystallography. Subsequent structure refinements reveal no radiation-damage-induced structural changes for insulin crystals up to a dose of 565.6 kGy, even though the total diffraction power of the crystals has on average decreased to 19.1% of its initial value for the same dose. A decay of the diffracting power by half is observed for a dose of D1/2 = 147.5 ± 19.1 kGy, which is about 1/300 of the dose before crystals show a similar decay at cryogenic temperatures.

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Contributing Institute(s):

1. FS-Photon Science (FS-PS)
2. Experimentebetreuung PETRA III (FS-PE)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. PSI-FELLOW - International Fellowship Program on Materials & Matter, Energy & Environment, Human Health & Life-Sciences, and Accelerator Technology (290605) (290605)
3. VH-VI-403 - In-Situ Nano-Imaging of Biological and Chemical Processes (2015_IFV-VH-VI-403) (2015_IFV-VH-VI-403)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2016

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Database coverage:
; ; ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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