Journal Article

PUBDB-2016-01888

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

Pande, K.CFEL*DESY* ; Hutchison, C. D. M. ; Groenhof, G. ; Aquila, A.>Extern* ; Robinson, J. S. ; Tenboer, J. ; Basu, S.CFEL*DESY* ; Boutet, S.>Extern* ; DePonte, D. P.>Extern* ; Liang, M. ; White, T. A.CFEL*DESY* ; Zatsepin, N. A. ; Yefanov, O.CFEL*DESY* ; Morozov, D. ; Oberthuer, D.CFEL*>Extern* ; Gati, C.CFEL*>Extern*EMBL* ; Subramanian, G.>Extern* ; James, D. ; Zhao, Y.>Extern* ; Koralek, J. ; Brayshaw, J. ; Kupitz, C. ; Conrad, C. ; Roy-Chowdhury, S.>Extern* ; Coe, J. D. ; Metz, M.CFEL*>Extern* ; Xavier, P. L.CFEL*DESY* ; Grant, T. D. ; Koglin, J. E. ; Ketawala, G. ; Fromme, R.>Extern* ;  rajer, V. ; Henning, R. ; Spence, J. C. H. ; Ourmazd, A. ; Schwander, P. ; Weierstall, U.>Extern* ; Frank, M.>Extern* ; Fromme, P.>Extern* ; Barty, A.CFEL*DESY* ; Chapman, H. N.CFEL*DESY* ; Moffat, K. ; van Thor, J. J. ; Schmidt, M. (Corresponding author)>Extern*

2016
American Association for the Advancement of Science16205 Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/science.aad5081

Abstract: A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

Note: (c) AAAS. Post referee full text in progress (embargo 6 months from 06 May 2016).

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. NANOMEM - Membrane Protein Nanocrystallography (317079) (317079)
3. 05K13GU7 - Test eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05K13GU7) (BMBF-05K13GU7)
4. BMBF-05K14CHA - 05K14CHA - SyncFELmed: Teilprojekt 2 (BMBF-05K14CHA) (BMBF-05K14CHA)
5. VH-VI-419 - Dynamic Pathways in Multidimensional Landscapes (VH-VI-419) (VH-VI-419)
6. VH-GS-500 - PIER Helmholtz Graduate School (2015_IFV-VH-GS-500) (2015_IFV-VH-GS-500)

Experiment(s):

1. Experiments at CFEL
2. Measurement at external facility

Appears in the scientific report 2016

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; IF >= 30 ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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