Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

Pande, K.; van Thor, J. J.; rajer, V.; Henning, R.; Basu, S.; Zhao, Y.; Ourmazd, A.; Robinson, J. S.; Fromme, P.; Fromme, R.; Chapman, H. N.; Barty, A.; Xavier, P. L.; Grant, T. D.; James, D.; Frank, M.; DePonte, D. P.; Roy-Chowdhury, S.; Kupitz, C.; Zatsepin, N. A.; Hutchison, C. D. M.; Conrad, C.; Moffat, K.; Morozov, D.; Koralek, J.; Subramanian, G.; Yefanov, O.; Schwander, P.; Weierstall, U.; Oberthuer, D.; Aquila, A.; Spence, J. C. H.; Tenboer, J.; Liang, M.; Groenhof, G.; Schmidt, M.; Ketawala, G.; Coe, J. D.; Metz, M.; Brayshaw, J.; Boutet, S.; White, T. A.; Koglin, J. E.; Gati, C.

doi:10.1126/science.aad5081

TY  - JOUR
AU  - Pande, K.
AU  - Hutchison, C. D. M.
AU  - Groenhof, G.
AU  - Aquila, A.
AU  - Robinson, J. S.
AU  - Tenboer, J.
AU  - Basu, S.
AU  - Boutet, S.
AU  - DePonte, D. P.
AU  - Liang, M.
AU  - White, T. A.
AU  - Zatsepin, N. A.
AU  - Yefanov, O.
AU  - Morozov, D.
AU  - Oberthuer, D.
AU  - Gati, C.
AU  - Subramanian, G.
AU  - James, D.
AU  - Zhao, Y.
AU  - Koralek, J.
AU  - Brayshaw, J.
AU  - Kupitz, C.
AU  - Conrad, C.
AU  - Roy-Chowdhury, S.
AU  - Coe, J. D.
AU  - Metz, M.
AU  - Xavier, P. L.
AU  - Grant, T. D.
AU  - Koglin, J. E.
AU  - Ketawala, G.
AU  - Fromme, R.
AU  -  rajer, V.
AU  - Henning, R.
AU  - Spence, J. C. H.
AU  - Ourmazd, A.
AU  - Schwander, P.
AU  - Weierstall, U.
AU  - Frank, M.
AU  - Fromme, P.
AU  - Barty, A.
AU  - Chapman, H. N.
AU  - Moffat, K.
AU  - van Thor, J. J.
AU  - Schmidt, M.
TI  - Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein
JO  - Science
VL  - 352
IS  - 6286
SN  - 1095-9203
CY  - Washington, DC [u.a.]
PB  - American Association for the Advancement of Science16205
M1  - PUBDB-2016-01888
SP  - 725 - 729
PY  - 2016
N1  - (c) AAAS. Post referee full text in progress (embargo 6 months from 06 May 2016).
AB  - A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000375417100042
C6  - pmid:27151871
DO  - DOI:10.1126/science.aad5081
UR  - https://bib-pubdb1.desy.de/record/297495
ER  -

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