guest ::
| Home > Publications database > Intrinsic Thermodynamics and Structure Correlation of Benzenesulfonamides with a Pyrimidine Moiety Binding to Carbonic Anhydrases I, II, VII, XII, and XIII |
| Home > Publications database > Intrinsic Thermodynamics and Structure Correlation of Benzenesulfonamides with a Pyrimidine Moiety Binding to Carbonic Anhydrases I, II, VII, XII, and XIII |
| Journal Article | PUBDB-2015-00003 |
; ; ; ; ; ; ; ; ;
2014
PLoS
Lawrence, Kan.
This record in other databases: 
Please use a persistent id in citations: doi:10.1371/journal.pone.0114106
Abstract: The early stage of drug discovery is often based on selecting the highest affinitylead compound. To this end the structural and energetic characterization of thebinding reaction is important. The binding energetics can be resolved into enthalpicand entropic contributions to the binding Gibbs free energy. Most compoundbinding reactions are coupled to the absorption or release of protons by the proteinor the compound. A distinction between the observed and intrinsic parameters ofthe binding energetics requires the dissection of the protonation/deprotonationprocesses. Since only the intrinsic parameters can be correlated with molecularstructural perturbations associated with complex formation, it is these parametersthat are required for rational drug design. Carbonic anhydrase (CA) isoforms areimportant therapeutic targets to treat a range of disorders including glaucoma,obesity, epilepsy, and cancer. For effective treatment isoform-specific inhibitors areneeded. In this work we investigated the binding and protonation energetics ofsixteen [(2-pyrimidinylthio)acetyl]benzenesulfonamide CA inhibitors usingisothermal titration calorimetry and fluorescent thermal shift assay. The compoundswere built by combining four sulfonamide headgroups with four tailgroups yielding16 compounds. Their intrinsic binding thermodynamics showed the limitations ofthe functional group energetic additivity approach used in fragment-based drugdesign, especially at the level of enthalpies and entropies of binding. Combinedwith high resolution crystal structural data correlations were drawn between thechemical functional groups on selected inhibitors and intrinsic thermodynamicparameters of CA-inhibitor complex formation.
; 
; 
; 
; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record
|
The record appears in these collections: |
PDF
