Solution structure of subunit ($\gamma$ ($\gamma$1-204)) of the Mycobacterium tuberculosis F-ATP synthase and the unique loop of ($\gamma$165-178), representing a novel TB drug target

Priya, R.; Grueber, G.; Lim, J.; Manimekalai, M.; Rao, S. S.; Biukovic, G.

doi:10.1007/s10863-012-9486-4

Journal Article

Solution structure of subunit ($\gamma$ ($\gamma$1-204)) of the Mycobacterium tuberculosis F-ATP synthase and the unique loop of ($\gamma$165-178), representing a novel TB drug target

Priya, R. ; Biukovic, G. ; Manimekalai, M. ; Lim, J. ; Rao, S. S. ; Grueber, G. (Corresponding author) ; DESY

2013
Springer Science + Business Media B.V Dordrecht [u.a.]

Journal of bioenergetics and biomembranes 45, 1-9 (2013) [10.1007/s10863-012-9486-4]

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Please use a persistent id in citations: doi:10.1007/s10863-012-9486-4

Abstract: Tuberculosis, caused by the strain Mycobacterium tuberculosis, is in focus of interest due to the emergence of multi- and extensive drug-resistant TB strains. The F(1)F(O) ATP synthase is one of the essential enzymes in energy requirement of both proliferating aerobic and hypoxic dormant stage of mycobacterium life cycle, and therefore a potential TB drug target. Subunit γ of F-ATP synthases plays an important role in coupling and catalysis via conformational transitions of its N- and C-termini as well as the bottom segment of the globular domain of γ, which is in close proximity to the rotating and ion-pumping c-ring. Here we describe the first production, purification and low resolution solution structure of subunit γ (γ(1-204), Mtγ(1-204)) of the M. tuberculosis F-ATP synthase. Mtγ(1-204) is a pear-like shaped protein with a molecular weight of 23 ± 2 kDa. Protein sequence analysis of Mtγ revealed differences in the amino acid composition to γ subunits from other sources, in particular the presence of a unique stretch of 13 amino acid residues (Mtγ(165-178)). NMR studies showed that Mtγ(165-178) forms a loop of polar residues. Mtγ(165-178) has been aligned at the bottom of the globular domain of the Escherichia coli subunit γ, being in close vicinity to the polar residues R41, Q42, E44 and Q46 (M. tuberculosis nomenclature) of the c-ring. The putative role(s) of Mtγ(165-178) in coupling and as a potential drug target are discussed.

Keyword(s): Antitubercular Agents: chemistry (MeSH) ; Antitubercular Agents: therapeutic use (MeSH) ; Bacterial Proteins: antagonists & inhibitors (MeSH) ; Bacterial Proteins: chemistry (MeSH) ; Bacterial Proteins: genetics (MeSH) ; Bacterial Proteins: metabolism (MeSH) ; Catalysis (MeSH) ; Drug Delivery Systems (MeSH) ; Enzyme Inhibitors: chemistry (MeSH) ; Enzyme Inhibitors: therapeutic use (MeSH) ; Mycobacterium tuberculosis: enzymology (MeSH) ; Mycobacterium tuberculosis: genetics (MeSH) ; Nuclear Magnetic Resonance, Biomolecular (MeSH) ; Protein Structure, Secondary (MeSH) ; Protein Subunits: antagonists & inhibitors (MeSH) ; Protein Subunits: chemistry (MeSH) ; Protein Subunits: diagnostic use (MeSH) ; Protein Subunits: metabolism (MeSH) ; Proton-Translocating ATPases: antagonists & inhibitors (MeSH) ; Proton-Translocating ATPases: chemistry (MeSH) ; Proton-Translocating ATPases: genetics (MeSH) ; Proton-Translocating ATPases: metabolism (MeSH) ; Recombinant Proteins: antagonists & inhibitors (MeSH) ; Recombinant Proteins: chemistry (MeSH) ; Recombinant Proteins: genetics (MeSH) ; Recombinant Proteins: metabolism (MeSH) ; Tuberculosis: drug therapy (MeSH) ; Tuberculosis: enzymology (MeSH) ; Antitubercular Agents ; Bacterial Proteins ; Enzyme Inhibitors ; Protein Subunits ; Recombinant Proteins ; Proton-Translocating ATPases