| 001 | 142572 | ||
| 005 | 20250730151051.0 | ||
| 024 | 7 | _ | |a pmid:23104121 |2 pmid |
| 024 | 7 | _ | |a 10.1007/s10863-012-9486-4 |2 doi |
| 024 | 7 | _ | |a 1573-6881 |2 ISSN |
| 024 | 7 | _ | |a 0145-479X |2 ISSN |
| 024 | 7 | _ | |a WOS:000314899500011 |2 WOS |
| 024 | 7 | _ | |a openalex:W2277704780 |2 openalex |
| 037 | _ | _ | |a PHPPUBDB-25512 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Priya, R. |b 0 |
| 110 | 1 | _ | |a DESY |b European Molecular Biology Laboratory |
| 245 | _ | _ | |a Solution structure of subunit ($\gamma$ ($\gamma$1-204)) of the Mycobacterium tuberculosis F-ATP synthase and the unique loop of ($\gamma$165-178), representing a novel TB drug target |
| 260 | _ | _ | |a Dordrecht [u.a.] |c 2013 |b Springer Science + Business Media B.V |
| 300 | _ | _ | |a 1-9 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1524565479_1391 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 440 | _ | 0 | |a Journal of Bioenergetics and Biomembranes |0 PERI:(DE-600)1482010-9 |v 45 |x 0145-479X |
| 500 | _ | _ | |a © Springer Science+Business Media New York; Post referee fulltext in progress; Embargo 12 months from publication |3 Converted on 2013-05-30 09:57 |
| 500 | _ | _ | |3 Converted on 2013-06-21 19:21 |
| 520 | _ | _ | |a Tuberculosis, caused by the strain Mycobacterium tuberculosis, is in focus of interest due to the emergence of multi- and extensive drug-resistant TB strains. The F(1)F(O) ATP synthase is one of the essential enzymes in energy requirement of both proliferating aerobic and hypoxic dormant stage of mycobacterium life cycle, and therefore a potential TB drug target. Subunit γ of F-ATP synthases plays an important role in coupling and catalysis via conformational transitions of its N- and C-termini as well as the bottom segment of the globular domain of γ, which is in close proximity to the rotating and ion-pumping c-ring. Here we describe the first production, purification and low resolution solution structure of subunit γ (γ(1-204), Mtγ(1-204)) of the M. tuberculosis F-ATP synthase. Mtγ(1-204) is a pear-like shaped protein with a molecular weight of 23 ± 2 kDa. Protein sequence analysis of Mtγ revealed differences in the amino acid composition to γ subunits from other sources, in particular the presence of a unique stretch of 13 amino acid residues (Mtγ(165-178)). NMR studies showed that Mtγ(165-178) forms a loop of polar residues. Mtγ(165-178) has been aligned at the bottom of the globular domain of the Escherichia coli subunit γ, being in close vicinity to the polar residues R41, Q42, E44 and Q46 (M. tuberculosis nomenclature) of the c-ring. The putative role(s) of Mtγ(165-178) in coupling and as a potential drug target are discussed. |
| 536 | _ | _ | |0 G:(DE-H253)POF2-D1.2-20130405 |f POF II |x 0 |c POF2-54G13 |a DORIS Beamline D1.2 (POF2-54G13) |
| 588 | _ | _ | |a Dataset connected to Pubmed |
| 650 | _ | 7 | |a Antitubercular Agents |0 0 |2 NLM Chemicals |
| 650 | _ | 7 | |a Bacterial Proteins |0 0 |2 NLM Chemicals |
| 650 | _ | 7 | |a Enzyme Inhibitors |0 0 |2 NLM Chemicals |
| 650 | _ | 7 | |a Protein Subunits |0 0 |2 NLM Chemicals |
| 650 | _ | 7 | |a Recombinant Proteins |0 0 |2 NLM Chemicals |
| 650 | _ | 7 | |a Proton-Translocating ATPases |0 EC 3.6.3.14 |2 NLM Chemicals |
| 650 | _ | 2 | |a Antitubercular Agents: chemistry |2 MeSH |
| 650 | _ | 2 | |a Antitubercular Agents: therapeutic use |2 MeSH |
| 650 | _ | 2 | |a Bacterial Proteins: antagonists & inhibitors |2 MeSH |
| 650 | _ | 2 | |a Bacterial Proteins: chemistry |2 MeSH |
| 650 | _ | 2 | |a Bacterial Proteins: genetics |2 MeSH |
| 650 | _ | 2 | |a Bacterial Proteins: metabolism |2 MeSH |
| 650 | _ | 2 | |a Catalysis |2 MeSH |
| 650 | _ | 2 | |a Drug Delivery Systems |2 MeSH |
| 650 | _ | 2 | |a Enzyme Inhibitors: chemistry |2 MeSH |
| 650 | _ | 2 | |a Enzyme Inhibitors: therapeutic use |2 MeSH |
| 650 | _ | 2 | |a Mycobacterium tuberculosis: enzymology |2 MeSH |
| 650 | _ | 2 | |a Mycobacterium tuberculosis: genetics |2 MeSH |
| 650 | _ | 2 | |a Nuclear Magnetic Resonance, Biomolecular |2 MeSH |
| 650 | _ | 2 | |a Protein Structure, Secondary |2 MeSH |
| 650 | _ | 2 | |a Protein Subunits: antagonists & inhibitors |2 MeSH |
| 650 | _ | 2 | |a Protein Subunits: chemistry |2 MeSH |
| 650 | _ | 2 | |a Protein Subunits: diagnostic use |2 MeSH |
| 650 | _ | 2 | |a Protein Subunits: metabolism |2 MeSH |
| 650 | _ | 2 | |a Proton-Translocating ATPases: antagonists & inhibitors |2 MeSH |
| 650 | _ | 2 | |a Proton-Translocating ATPases: chemistry |2 MeSH |
| 650 | _ | 2 | |a Proton-Translocating ATPases: genetics |2 MeSH |
| 650 | _ | 2 | |a Proton-Translocating ATPases: metabolism |2 MeSH |
| 650 | _ | 2 | |a Recombinant Proteins: antagonists & inhibitors |2 MeSH |
| 650 | _ | 2 | |a Recombinant Proteins: chemistry |2 MeSH |
| 650 | _ | 2 | |a Recombinant Proteins: genetics |2 MeSH |
| 650 | _ | 2 | |a Recombinant Proteins: metabolism |2 MeSH |
| 650 | _ | 2 | |a Tuberculosis: drug therapy |2 MeSH |
| 650 | _ | 2 | |a Tuberculosis: enzymology |2 MeSH |
| 693 | _ | _ | |a DORIS III |f DORIS Beamline D1.2 |1 EXP:(DE-H253)DORISIII-20150101 |0 EXP:(DE-H253)D-D1.2-20150101 |6 EXP:(DE-H253)D-D1.2-20150101 |x 0 |
| 700 | 1 | _ | |a Biukovic, G. |b 1 |
| 700 | 1 | _ | |a Manimekalai, M. |b 2 |
| 700 | 1 | _ | |a Lim, J. |b 3 |
| 700 | 1 | _ | |a Rao, S. S. |b 4 |
| 700 | 1 | _ | |a Grueber, G. |0 P:(DE-HGF)0 |b 5 |e Corresponding author |
| 773 | _ | _ | |a 10.1007/s10863-012-9486-4 |g Vol. 45, p. 1-9 |0 PERI:(DE-600)1482010-9 |q 45<1-9 |p 1-9 |t Journal of bioenergetics and biomembranes |v 45 |y 2013 |x 0145-479X |
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| 913 | 1 | _ | |b Struktur der Materie |1 G:(DE-HGF)POF2-540 |0 G:(DE-HGF)POF2-54G13 |2 G:(DE-HGF)POF2-500 |v DORIS III |9 G:(DE-H253)POF2-D1.2-20130405 |x 0 |a DE-H253 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF2 |l Forschung mit Photonen, Neutronen, Ionen |
| 914 | 1 | _ | |a not yet published |y 2013 |
| 915 | _ | _ | |a Medline |0 StatID:(DE-HGF)0300 |2 StatID |
| 915 | _ | _ | |a No Author Disambiguation |0 StatID:(DE-HGF)1 |2 StatID |
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