Journal Article

PHPPUBDB-24709

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The DNA-DNA spacing in gemini surfactants-DOPE-DNA complexes

Pullmannova, P. ; Funari, S. S. ; Devinsky, F. ; Uhrikova, D. ; DESY

2012
Elsevier Amsterdam

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Please use a persistent id in citations: doi:10.1016/j.bbamem.2012.05.021

Abstract: Gemini surfactants from the homologous series of alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide) (CnCS12, number of spacer carbons n=2-12) and dioleoylphosphatidylethanolamine (DOPE) were used for cationic liposome (CL) preparation. CLs condense highly polymerized DNA creating complexes. Small-angle X-ray diffraction identified them as condensed lamellar phase L(α)(C) in the studied range of molar ratios CnGS12/DOPE in the temperature range 20-60°C. The DNA-DNA distance (d(DNA)) is studied in dependence to CnGS12 spacer length and membrane surface charge density. The high membrane surface charge densities (CnGS12/DOPE=0.35 and 0.4mol/mol) lead to the linear dependence of d(DNA) vs. n correlating with the interfacial area of the CnGS12 molecule.

Keyword(s): DNA: chemistry (MeSH) ; Phosphatidylethanolamines: chemistry (MeSH) ; Scattering, Small Angle (MeSH) ; Surface-Active Agents: chemistry (MeSH) ; X-Ray Diffraction (MeSH) ; 1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine ; Phosphatidylethanolamines ; Surface-Active Agents ; DNA

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Contributing Institute(s):

1. Experiments with synchrotron radiation (HASYLAB)

Research Program(s):

1. DORIS Beamline A2 (POF2-54G13) (POF2-54G13)
2. FS-Proposal: II-20100372 EC (II-20100372-EC) (II-20100372-EC)

Experiment(s):

1. DORIS Beamline A2 (DORIS III)

Appears in the scientific report 2012

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Database coverage:
; No Author Disambiguation

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