TY  - JOUR
AU  - Pullmannova, P.
AU  - Funari, S. S.
AU  - Devinsky, F.
AU  - Uhrikova, D.
AU  - DESY
TI  - The DNA-DNA spacing in gemini surfactants-DOPE-DNA complexes
JO  - Biochimica et biophysica acta / Biomembranes
VL  - 1818
SN  - 0005-2736
CY  - Amsterdam
PB  - Elsevier
M1  - PHPPUBDB-24709
SP  - 2725-2731
PY  - 2012
AB  - Gemini surfactants from the homologous series of alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide) (CnCS12, number of spacer carbons n=2-12) and dioleoylphosphatidylethanolamine (DOPE) were used for cationic liposome (CL) preparation. CLs condense highly polymerized DNA creating complexes. Small-angle X-ray diffraction identified them as condensed lamellar phase L(α)(C) in the studied range of molar ratios CnGS12/DOPE in the temperature range 20-60°C. The DNA-DNA distance (d(DNA)) is studied in dependence to CnGS12 spacer length and membrane surface charge density. The high membrane surface charge densities (CnGS12/DOPE=0.35 and 0.4mol/mol) lead to the linear dependence of d(DNA) vs. n correlating with the interfacial area of the CnGS12 molecule.
KW  - DNA: chemistry
KW  - Phosphatidylethanolamines: chemistry
KW  - Scattering, Small Angle
KW  - Surface-Active Agents: chemistry
KW  - X-Ray Diffraction
KW  - 1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine (NLM Chemicals)
KW  - Phosphatidylethanolamines (NLM Chemicals)
KW  - Surface-Active Agents (NLM Chemicals)
KW  - DNA (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:22634275
UR  - <Go to ISI:>//WOS:000309081700026
DO  - DOI:10.1016/j.bbamem.2012.05.021
UR  - https://bib-pubdb1.desy.de/record/140072
ER  -