Journal Article

PUBDB-2022-00394

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure

Schroer, M. A. (Corresponding author)Extern*EMBL* ; Schewa, S.Extern*EMBL* ; Gruzinov, A. Y.EMBL* ; Rönnau, C.Extern*EMBL* ; Lahey-Rudolph, J. M.Extern*XFEL.EU*EMBL* ; Blanchet, C. E.Extern*EMBL* ; Zickmantel, T.Extern*EMBL* ; Song, Y.-H.Extern*EMBL* ; Svergun, D. I.EMBL* ; Roessle, M.Extern*EMBL*

2021
Macmillan Publishers Limited, part of Springer Nature [London]

This record in other databases:        

Please use a persistent id in citations: doi:10.1038/s41598-021-01774-6  doi:10.3204/PUBDB-2022-00394

Abstract: During the last decades discussions were taking place on the existence of global, non-thermal structural changes in biological macromolecules induced by Terahertz (THz) radiation. Despite numerous studies, a clear experimental proof of this effect for biological particles in solution is still missing. We developed a setup combining THz-irradiation with small angle X-ray scattering (SAXS), which is a sensitive method for detecting the expected structural changes. We investigated in detail protein systems with different shape morphologies (bovine serum albumin, microtubules), which have been proposed to be susceptible to THz-radiation, under variable parameters (THz wavelength, THz power densities up to 6.8 mW/cm$^2$, protein concentrations). None of the studied systems and conditions revealed structural changes detectable by SAXS suggesting that the expected non-thermal THz-induced effects do not lead to alterations of the overall structures, which are revealed by scattering from dissolved macromolecules. This leaves us with the conclusion that, if such effects are present, these are either local or outside of the spectrum and power range covered by the present study.

---

Contributing Institute(s):

1. EMBL (EMBL)
2. EMBL-User (EMBL-User)
3. DOOR-User (DOOR ; HAS-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)

Experiment(s):

1. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2021

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF < 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---