Journal Article

DESY-2014-02447

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Solution Small Angle X-ray Scattering (SAXS) Studies of RecQfrom Deinococcus radiodurans and Its Complexes withJunction DNA Substrates

Wang, W.Extern*>EMBL* ; Du, Q. ; Zhang, W. ; Liu, G.Extern*>EMBL* ; Shtykova, E.Extern* ; Xu, J. ; Liu, P. ; Dong, Y.

2013
Soc. Bethesda, Md.

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Please use a persistent id in citations: doi:10.1074/jbc.M113.502112  doi:10.3204/DESY-2014-02447

Abstract: RecQ helicases, essential enzymes for maintaining genome integrity, possess the capability to participate in a wide variety of DNA metabolisms. They can initiate the homologous recombination repair pathway by unwinding damaged dsDNA and suppress hyper-recombination by promoting Holliday junction (HJ) migration. To learn how DrRecQ participates in the homologous recombination repair pathway, solution structures of Deinococcus radiodurans RecQ (DrRecQ) and its complexes with DNA substrates were investigated by small angle x-ray scattering. We found that the catalytic core and the most N-terminal HRDC (helicase and RNase D C-terminal) domain (HRDC1) undergo a conformational change to a compact state upon binding to a junction DNA. Furthermore, models of DrRecQ in complexes with two kinds of junction DNA (fork junction and HJ) were built based on the small angle x-ray scattering data, and together with the EMSA results, possible binding sites were proposed. It is demonstrated that two DrRecQ molecules bind to the opposite arms of HJ. This architecture is similar to the RuvAB complex and is hypothesized to be highly conserved in the other HJ migration proteins. This work provides us new clues to understand the roles DrRecQ plays in the RecFOR pathway.

Note: © The American Society for Biochemistry and Molecular Biology, Inc.

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Contributing Institute(s):

1. EMBL-User (EMBL-User)
2. EMBL (EMBL)

Research Program(s):

1. PETRA Beamline P12 (POF2-54G14) (POF2-54G14)

Experiment(s):

1. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2013

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Database coverage:
; ; BIOSIS Previews ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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