Journal Article

PHPPUBDB-12653

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structure of the X (ADRP) domain of nsp3 from feline coronavirus

Wojdyla, J. A. ; Manolaridis, I. ; Snijder, E. J. ; Gorbalenya, A. E. ; Coutard, B. ; Piotrowski, Y. ; Hilgenfeld, R. ; Tucker, P. A. (Corresponding author) ; DESY

2009
Munksgaard Copenhagen

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Please use a persistent id in citations: doi:10.1107/S0907444909040074

Abstract: The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 A resolution, respectively. In the tetragonal crystal form, glycerol-3-phosphate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 A resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.

Keyword(s): Adenosine Diphosphate Ribose: chemistry (MeSH) ; Adenosine Diphosphate Ribose: metabolism (MeSH) ; Amino Acid Sequence (MeSH) ; Binding Sites (MeSH) ; Coronavirus, Feline: enzymology (MeSH) ; Crystallography, X-Ray (MeSH) ; Glycerophosphates: chemistry (MeSH) ; Glycerophosphates: metabolism (MeSH) ; Molecular Sequence Data (MeSH) ; Protein Binding (MeSH) ; Protein Interaction Domains and Motifs (MeSH) ; RNA Replicase: chemistry (MeSH) ; Sequence Alignment (MeSH) ; Sequence Homology, Amino Acid (MeSH) ; Viral Nonstructural Proteins: chemistry (MeSH) ; Glycerophosphates ; Viral Nonstructural Proteins ; Adenosine Diphosphate Ribose ; alpha-glycerophosphoric acid ; RNA Replicase

Note: © International Union of Crystallography; Post referee fulltext in progress 2

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Contributing Institute(s):

1. EMBL (EMBL(-2012))

Research Program(s):

1. FS Beamline without reference (POF1-550) (POF1-550)

Experiment(s):

1. Unknown DESY Beamline

Appears in the scientific report 2009

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Database coverage:
; JCR ; No Author Disambiguation ; No Authors Fulltext ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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