Contribution to a book PUBDB-2024-05660

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Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography

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2024
Springer US New York, NY
ISBN: 978-1-0716-3925-2, 978-1-0716-3926-9 (electronic)

[Ebook] Rift Valley Fever Virus : Methods and Protocols / Lozach, Pierre-Yves 1st ed. 2024, New York, NY : Springer US, 2024, New York, NY : Springer US, Methods in Molecular Biology 2824, : 1st ed. 2024, 221 - 239 () [10.1007/978-1-0716-3926-9_15]  GO

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Abstract: An Introduction to Rift Valley Fever Virus -- Aedes aegypti Vector Competence Assay for Rift Valley Fever Virus using Artificial Blood Meal -- Isolation of Rift Valley Fever Virus from Field Specimens using Cell Culture Approaches -- Detection and Diagnosis of Rift Valley Fever Virus -- Analysis of Negative-Strand RNA Viruses by RT-qPCR: Rift Valley Fever Virus and Toscana Virus -- Validated Methods for Effective Decontamination and Inactivation of Rift Valley Fever Virus -- Guidelines for In Vitro Production and Quantification of Rift Valley Fever Virus -- Cell-Free Synthesis of Bunyavirales Proteins in View of Their Structural Characterization by Nuclear Magnetic Resonance -- Expression and Purification of RVFV Glycoprotein C for Structural Studies using Baculovirus Expression System -- Next-Generation Sequencing and Proteomics-Enabled Approach for Rapid and High-Throughput Isolation of Virus-Neutralizing Nanobodies -- Use of Single-Domain Antibodies Against the NSm Protein for the Detection of Cells Infected by Rift Valley Fever Virus -- Making Rift Valley Fever Viral Particles Fluorescent -- Identification of RVFV Host Factors using Quantitative Interaction Proteomics -- Genome-Wide CRISPR-Cas9 Screening for the Identification of Host Dependency Factors of Emerging Viruses -- Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography -- Imaging Bunyavirus Infections by Transmission Electron Microscopy: Conventional Sample Preparation vs High-Pressure Freezing and Freeze-Substitution -- The Phlebovirus Ribonucleoprotein: An Overview -- Nucleotide Resolution Mapping of Rift Valley Fever Virus Nucleoprotein - Genome RNA Interactions -- Characterization of RVFV Nucleocapsid Protein Binding Sites on RNA by iCLIP-seq -- Yeast Expression and Activity Assay of RVFV Polymerase -- Single-Molecule Visualization of Bunyavirus Genome Segments using Fluorescence In Situ Hybridization -- Single-Cell RNA-Sequencing of RVFV Infection -- Using RNAseq to Uncover Transcriptional and Splicing Differences in Host Cells during Rift Valley Fever Virus Infection -- Quantification of RVFV Specific T Cell Responses in Mice Pre-Immunized with Potential Vaccine Candidates -- Use of Human Macrophages to Study Bunyavirus NSs Functions -- 3D Spheroid and Organoid Models to Study Neuroinfection of RNA Viruses -- Laboratory Animal Models for Rift Valley Fever Virus Disease -- Monitoring RVFV Infection using Bioluminescent Reporter Viruses In Vivo

Keyword(s): Virology (LCSH)

Classification:

Contributing Institute(s):
  1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald (CSSB-LIV-KG)
Research Program(s):
  1. 899 - ohne Topic (POF4-899) (POF4-899)
Experiment(s):
  1. No specific instrument

Appears in the scientific report 2024
Database coverage:
Medline ; SCOPUS
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 Record created 2024-08-30, last modified 2025-07-23



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