Preprint

PUBDB-2024-00739

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Reconstitution of a Biofilm Adhesin System from a Sulfate-Reducing Bacterium in Pseudomonas fluorescens

Karbelkar, A. A. ; Font, M.Extern*DESY* ; Smith, T. J. ; Sondermann, H.CSSB*DESY* ; O’Toole, G. A. (Corresponding author)

2023

This record in other databases:    

Please use a persistent id in citations: doi:10.1101/2023.11.22.568322  doi:10.3204/PUBDB-2024-00739

Abstract: Biofilms of the sulfate reducing bacterium (SRB) Desulfovibrio vulgaris Hildenborough (DvH) can facilitate metal corrosion in various industrial and environmental settings leading to substantial economic losses; however, the mechanisms of biofilm formation by DvH are not yet well-understood. Evidence suggests that a large adhesin, DvhA, may be contributing to biofilm formation in DvH. The dvhA gene and its neighbors encode proteins that resemble the Lap system, which regulates biofilm formation by Pseudomonas fluorescens, including a LapG-like protease DvhG and effector protein DvhD, which has key differences from the previously described LapD. By expressing the Lap-like adhesion components of DvH in P. fluorescens, our data support the model that the N-terminal fragment of the large adhesin DvhA serves as an adhesin “retention module” and is the target of the DvhG/DvhD regulatory module, thereby controlling cell-surface location of the adhesin. By heterologously expressing the DvhG/DvhD-like proteins in a P. fluorescens background lacking native regulation (ΔlapGΔlapD) we also show that cell surface regulation of the adhesin is dependent upon the intracellular levels of c-di-GMP. This study provides insight into the key players responsible for biofilm formation by DvH, thereby expanding our understanding of Lap-like systems.

---

Contributing Institute(s):

1. Strukturelle Mikrobiologie CSSB (CSSB-DESY-HS)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2024

---

Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; National-Konsortium ; Published ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---

---

Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Karbelkar, A. A. ; Font, M.Extern* ; Smith, T. J. ; Sondermann, H.CSSB*DESY* ; O’Toole, G. A. (Corresponding author)
Reconstitution of a biofilm adhesin system from a sulfate-reducing bacterium in Pseudomonas fluorescens
Proceedings of the National Academy of Sciences of the United States of America 121(13), e2320410121 (2024) [10.1073/pnas.2320410121]2024     Files BibTeX | EndNote: XML, Text | RIS

---