Journal Article

PUBDB-2024-00511

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Alpha-lactalbumin amyloid-like fibrils for intestinal delivery: Formation, physiochemical characterization, and digestive fate of capsaicin-loaded fibrils

Romano, A. ; Engelberg, Y. ; Landau, M.CSSB*EMBL*DESY* ; Lesmes, U. (Corresponding author)

2023
Elsevier Amsterdam

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Please use a persistent id in citations: doi:10.1016/j.foodhyd.2022.108248

Abstract: Engineering protein architectures offers numerous opportunities to deliver consumers with added values yet mandates careful screening of possible risks. This study explores the fabrication of bovine alpha-lactalbumin (ALA) amyloid-like fibrils (AF) for gastro-intestinal delivery of capsaicin (CAP), a pungent compound which possesses health beneficial virtues. Acidic incubation of ALA with CAP accelerates formation of ALA-AF, as confirmed by transmission electron microscope, with a size increase from 3.7 ± 0.4 nm to 175 ± 58 nm and a melting temperature shift from T$_m$ = 61.8 °C to T$_m$ = 89.9 °C for native ALA and ALA-AF entrapping 200 μM CAP, respectively. In addition to encapsulation efficiency and loading capacity measurements, this work provides evidence that fibrillation attenuates the in vitro digestive proteolysis of ALA and diminishes the levels of bioaccessible bioactive peptides by an order of magnitude compared to native ALA. This is accompanied by sustained release of 7.8 ± 1.7% to 55.7 ± 12.3% CAP under gastric and intestinal conditions. Overall, this work presents a new possible avenue for designing protein structures to entrap bioactive moieties along-side a call to interrogate the possible ramifications to protein digestion and consumer health.

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Contributing Institute(s):

1. FPF Functional Protein Fibrils (CSSB-F)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. No specific instrument

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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