Poster

PUBDB-2023-01305

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Systems and 3D imaging approaches to understand the Aspergillus niger chitin synthase gene repertoire

Barthel, L. ; Duda, S. ; Mueller, H.TUM* ; Hammel, J.HZG*Hereon* ; Briesen, H. (Corresponding author)TUM* ; Meyer, V.

2023

Abstract: The filamentous fungus Aspergillus niger is broadly applied as a cell factory for the production of proteins, enzymes and organic acids. In all filamentous fungi, hyphal growth and (protein) secretion are strictly coupled processes. One organelle affecting both processes is the fungal cell wall with chitin as one of its main components. In this project, we aimed to understand the role of nine predicted chitin synthase genes on the growth, morphology and productivity of Aspergillus niger. We therefore constructed a chitin synthase knock-out library and compared the phenotypes of all mutant strains during solid and submerged cultivations with its parental wild-type strain. Corresponding data will be shown including transcriptomic data, microscopic and macroscopic data based on 2D and 3D synchrotron X-ray microtomography analyses as well as data addressing the cell wall stress responsiveness of all strains.

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)
2. Helmholtz-Zentrum Hereon (Hereon)
3. Technische Universität München (TUM)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. FS-Proposal: I-20211150 (I-20211150) (I-20211150)

Experiment(s):

1. PETRA Beamline P05 (PETRA III)

Appears in the scientific report 2023

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