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001     580545
005     20230322214110.0
037 _ _ |a PUBDB-2023-01305
041 _ _ |a German
100 1 _ |a Barthel, Lars
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111 2 _ |a 16th European Conference on Fungal Genetics
|g ECFG16
|c Innsbruck
|d 2023-03-05 - 2023-03-08
|w Germany
245 _ _ |a Systems and 3D imaging approaches to understand the Aspergillus niger chitin synthase gene repertoire
260 _ _ |c 2023
336 7 _ |a Conference Paper
|0 33
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520 _ _ |a The filamentous fungus Aspergillus niger is broadly applied as a cell factory for the production of proteins, enzymes and organic acids. In all filamentous fungi, hyphal growth and (protein) secretion are strictly coupled processes. One organelle affecting both processes is the fungal cell wall with chitin as one of its main components. In this project, we aimed to understand the role of nine predicted chitin synthase genes on the growth, morphology and productivity of Aspergillus niger. We therefore constructed a chitin synthase knock-out library and compared the phenotypes of all mutant strains during solid and submerged cultivations with its parental wild-type strain. Corresponding data will be shown including transcriptomic data, microscopic and macroscopic data based on 2D and 3D synchrotron X-ray microtomography analyses as well as data addressing the cell wall stress responsiveness of all strains.
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700 1 _ |a Duda, Sven
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700 1 _ |a Mueller, Henri
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700 1 _ |a Hammel, Joerg
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700 1 _ |a Briesen, Heiko
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|e Corresponding author
700 1 _ |a Meyer, Vera
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909 C O |o oai:bib-pubdb1.desy.de:580545
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910 1 _ |a Technische Universität Berlin
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910 1 _ |a Technische Universität Berlin
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910 1 _ |a Technische Universität München
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910 1 _ |a Technische Universität München
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910 1 _ |a Technische Universität Berlin
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914 1 _ |y 2023
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