Journal Article

PUBDB-2022-07773

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nickel distribution in Stackhousia tryonii shown by synchrotron X-ray fluorescence micro-computed tomography

Van Der Ent, A. (Corresponding author)Extern* ; Spiers, K.DESY* ; Brueckner, D.DESY* ; Erskine, P. D.Extern*

2022
CSIRO Publ. Collingwood Victoria

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Please use a persistent id in citations: doi:10.1071/BT22012  doi:10.3204/PUBDB-2022-07773

Abstract: Context: Hyperaccumulator plants are of considerable interest for their extreme physiology. Stackhousia tryonii is a nickel (Ni) hyperaccumulator plant endemic to ultramafic outcrops in Queensland (Australia) capable of attaining up to 41 300 μg g−1 foliar Ni.Aims: This study sought to elucidate the distribution of Ni in S. tryonii by using synchrotron X-ray fluorescence micro-computed tomography (XFM-CT), complemented with elemental maps acquired from physically sectioned plant organs. Its Ni-enriched cylindrical photosynthetic stems make them particularly well suited samples for synchrotron XFM-CT.Methods: XFM-CT enables ‘virtual sectioning’ of a sample, avoiding artefacts arising from physical sample preparation. The method can be used on fresh samples that are frozen during the analysis, which preserves ‘life-like’ conditions by limiting radiation damage. It also prevents/minimises other artefacts.Key results: The results showed that Ni is mainly concentrated in the apoplastic space surrounding epidermal cells, and in some epidermal cell vacuoles. This finding is significant because this ‘free’ solute Ni is likely to be lost during physical sectioning.Conclusions and implications: This case study has highlighted the utility of the XFM-CT approach for visualising metals within intact plant organs, which may be used across the plant sciences.

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)
2. Experimentebetreuung PETRA III (FS-PET-S)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. FS-Proposal: I-20190028 (I-20190028) (I-20190028)

Experiment(s):

1. PETRA Beamline P06 (PETRA III)

Appears in the scientific report 2022

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Database coverage:
; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Essential Science Indicators ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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