Journal Article

PUBDB-2021-01791

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison

Mehrabi, P.CFEL*MPG*EMBL*Extern* ; Bücker, R.CFEL*CSSB* ; Bourenkov, G.EMBL* ; Ginn, H. M. ; von Stetten, D. ; Müller-Werkmeister, H. M. ; Kuo, A. ; Morizumi, T. ; Eger, B. T. ; Ou, W.-L. ; Oghbaey, S. ; Sarracini, A. ; Besaw, J. E. ; Pare´-Labrosse, O. ; Meier, S. ; Schikora, H.CFEL*MPG* ; Tellkamp, F.CFEL*MPG* ; Marx, A. ; Sherrell, D. A. ; Axford, D. ; Owen, R. L. ; Ernst, O. P. ; Pai, E. F. ; Schulz, E. C. ; Miller, R. J. D. (Corresponding author)CFEL*MPG*

2021
Assoc. Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/sciadv.abf1380  doi:10.3204/PUBDB-2021-01791

Abstract: For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins, reasonable data statistics can be obtained with approximately 5000 room-temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that the quality of diffraction data obtained from these samples is linked to the properties of the crystals rather than to the radiation source. Therefore, for other systems with similar properties, time-resolved experiments can be conducted at the radiation source that best matches the desired time resolution.

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Contributing Institute(s):

1. Forschungsgruppe für strukturelle Dynamik (MPSD)
2. MPSD (CFEL-ARD)
3. MPSD (CFEL-SSUMP)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)

Experiment(s):

1. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2021

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