Journal Article

PUBDB-2019-03664

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Virus Structures by X-Ray Free-Electron Lasers

Meents, A. (Corresponding author)CFEL*DESY* ; Wiedorn, M. O.CFEL*XFEL.EU*

2019
Annual Reviews Palo Alto, Calif.

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Please use a persistent id in citations: doi:10.1146/annurev-virology-092818-015724

Abstract: Until recently X-ray crystallography has been the standard technique forvirus structure determinations. Available X-ray sources have continuouslyimproved over the decades, leading to the realization of X-ray free-electronlasers (XFELs). They provide high-intensity femtosecond X-ray pulses,which allow for new kinds of experiments by making use of the diffractionbefore-destruction principle. By overcoming classical dose constraints, theyat least in principle allow researchers to perform X-ray virus structuredetermination for single particles at room temperature. Simultaneously,the availability of XFELs led to the development of the method of serialfemtosecond crystallography, where a crystal structure is determined fromthe measurement of hundreds to thousands of microcrystals. In the case ofvirus crystallography this method does not require freezing of the crystalsand allows researchers to perform experiments under non-equilibriumconditions (e.g., by laser-induced temperature jumps or rapid chemicalmixing), which is currently not possible with electron microscopy.

Note: © by Annual Reviews.; Post referee fulltext in progress; Embargo 12 months from publication

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Contributing Institute(s):

1. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
2. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. 6G2 - FLASH (POF3-622) (POF3-622)

Experiment(s):

1. FLASH Beamline BL2 (FLASH)
2. Experiments at CFEL

Appears in the scientific report 2019

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Database coverage:
; BIOSIS Previews ; BIOSIS Reviews Reports And Meetings ; Clarivate Analytics Master Journal List ; IF >= 5 ; JCR ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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