Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Kang, Yanyong; Jiang, Hualiang; Caffrey, Martin; Gao, Xiang; Yang, Huaiyu; Standfuss, Jörg; Grotjohann, Ingo; Spence, John C. H.; Hubbell, Wayne L.; West, Graham M.; Gu, Xin; Li, Dianfan; Caro, Lydia N.; Van Eps, Ned; Zatsepin, Nadia A.; Fromme, Raimund; White, Thomas; Moeller, Arne; Wang, Meitian; Ke, Jiyuan; Howe, Nicole; Potter, Clinton S.; Dong, Yuhui; Liu, Haiguang; Yefanov, Oleksandr; Carragher, Bridget; Gurevich, Vsevolod V.; Weierstall, Uwe; Jiang, Yi; de Waal, Parker W.; Messerschmidt, Marc; Cherezov, Vadim; Ishchenko, Andrii; Griffin, Patrick R.; Liu, Wei; Tan, Minjia; Roy-Chowdhury, Shatabdi; Pascal, Bruce D.; Zhang, Chenghai; Pal, Kuntal; Han, Gye Won; Zhao, Yingming; Ernst, Oliver P.; Chapman, Henry N.; Williams, Garth J.; Gati, Cornelius; Zheng, Zhong; He, Yuanzheng; Diederichs, Kay; Wang, Dingjie; Tan, M. H. Eileen; Barty, Anton; Kupitz, Christopher; Boutet, Sébastien; Xu, Qingping; Vishnivetskiy, Sergey A.; Lisova, Stella; Zhou, X. Edward; Li, Jun; Fromme, Petra; Conrad, Chelsie E.; James, Daniel; Stevens, Raymond C.; Xu, H. Eric; Katritch, Vsevolod; Lee, Regina J.; Ma, Jinming; Suino-Powell, Kelly M.; Basu, Shibom; Melcher, Karsten; Zhi, Xiaoyong; Coe, Jesse

doi:10.1038/nature14656

Journal Article

PUBDB-2015-04295

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Kang, Y. ; Zhou, X. E. ; Gao, X. ; He, Y. ; Liu, W. ; Ishchenko, A. ; Barty, A.CFEL*DESY* ; White, T.CFEL*DESY* ; Yefanov, O.CFEL*DESY* ; Han, G. W. ; Xu, Q. ; de Waal, P. W. ; Ke, J. ; Tan, M. H. E. ; Zhang, C. ; Moeller, A. ; West, G. M. ; Pascal, B. D. ; Van Eps, N. ; Caro, L. N. ; Vishnivetskiy, S. A. ; Lee, R. J. ; Suino-Powell, K. M. ; Gu, X. ; Pal, K. ; Ma, J. ; Zhi, X. ; Boutet, S.>Extern* ; Williams, G. J. ; Messerschmidt, M.>Extern*XFEL* ; Gati, C.CFEL* ; Zatsepin, N. A. ; Wang, D. ; James, D. ; Basu, S. ; Roy-Chowdhury, S. ; Conrad, C. E. ; Coe, J.>Extern* ; Liu, H. ; Lisova, S. ; Kupitz, C. ; Grotjohann, I. ; Fromme, R.>Extern* ; Jiang, Y. ; Tan, M. ; Yang, H. ; Li, J.>Extern* ; Wang, M. ; Zheng, Z. ; Li, D. ; Howe, N. ; Zhao, Y. ; Standfuss, J. ; Diederichs, K. ; Dong, Y. ; Potter, C. S. ; Carragher, B. ; Caffrey, M. ; Jiang, H. ; Chapman, H. N.CFEL*DESY* ; Spence, J. C. H. ; Fromme, P.>Extern* ; Weierstall, U.>Extern* ; Ernst, O. P. ; Katritch, V. ; Gurevich, V. V. ; Griffin, P. R. ; Hubbell, W. L. ; Stevens, R. C. ; Cherezov, V. ; Melcher, K. ; Xu, H. E. (Corresponding author)

2015
Macmillan28177 London

Nature 523(7562), 561 - 567 (2015) [10.1038/nature14656]

This record in other databases:

Please use a persistent id in citations: doi:10.1038/nature14656

Abstract: G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin–arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ~20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology.

Classification: