Journal Article

PUBDB-2015-01178

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Cloning, Overexpression, Purification and Preliminary X-Ray Analysis of the Protein Kinase Domain of Enhanced Disease Resistance 1 (EDR1) from Arabidopsis Thaliana

Kaljunen, H. (Corresponding Author)>Extern*EMBL* ; Panneerselvam, S.DESY* ; Mueller-Dieckmann, J.

2014
Blackwell Oxford [u.a.]

This record in other databases:        

Please use a persistent id in citations: doi:10.1107/S2053230X14011844

Abstract: Enhanced disease resistance 1 is a member of the Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK) family that negatively regulates disease resistance, ethylene-induced senescence and programmed cell death in response to both abiotic and biotic stresses. A catalytically inactive form of the EDR1 kinase domain was successfully cloned, expressed, purified and crystallized. Crystallization was conducted in the presence of the ATP analogue AMP-PNP. The crystals belonged to space group $P3_{2}21$ and contained two molecules in the asymmetric unit. The crystals diffracted X-rays to 2.55 Å resolution.

Note: (c) International Union of Crystallography. Post referee full text in progress.

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Contributing Institute(s):

1. EMBL (EMBL)
2. Experimentebetreuung PETRA III (FS-PE)

Research Program(s):

1. PETRA Beamline P13 (POF2-54G14) (POF2-54G14)

Experiment(s):

1. PETRA Beamline P13 (PETRA III)

Appears in the scientific report 2014

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Database coverage:
; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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