TY - JOUR AU - Kaljunen, Heidi AU - Panneerselvam, Saravanan AU - Mueller-Dieckmann, Jochen TI - Cloning, Overexpression, Purification and Preliminary X-Ray Analysis of the Protein Kinase Domain of Enhanced Disease Resistance 1 (EDR1) from Arabidopsis Thaliana JO - Acta crystallographica / F VL - 70 IS - 7 SN - 1744-3091 CY - Oxford [u.a.] PB - Blackwell M1 - PUBDB-2015-01178 SP - 959-962 PY - 2014 N1 - (c) International Union of Crystallography. Post referee full text in progress. AB - Enhanced disease resistance 1 is a member of the Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK) family that negatively regulates disease resistance, ethylene-induced senescence and programmed cell death in response to both abiotic and biotic stresses. A catalytically inactive form of the EDR1 kinase domain was successfully cloned, expressed, purified and crystallized. Crystallization was conducted in the presence of the ATP analogue AMP-PNP. The crystals belonged to space group P3<sub>2</sub>21 and contained two molecules in the asymmetric unit. The crystals diffracted X-rays to 2.55 Å resolution. LB - PUB:(DE-HGF)16 UR - <Go to ISI:>//WOS:000338923200023 C6 - pmid:25005098 DO - DOI:10.1107/S2053230X14011844 UR - https://bib-pubdb1.desy.de/record/207194 ER -