Journal Article

DESY-2014-01386

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Benzenesulfonamides with pyrimidine moiety as inhibitors of human carbonic anhydrases I, II, VI, VII, XII, and XIII

Čapkauskaitė, E. ; Zubrienė, A. ; Smirnov, A.Extern* ; Torresan, J. ; Kišonaitė, M. ; Kazokaitė, J. ; Gylytė, J. ; Michailovienė, V. ; Jogaitė, V. ; Manakova, E.Extern* ; Gražulis, S.Extern* ; Tumkevičius, S. ; Matulis, D. (Corresponding author)

2013
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.bmc.2013.09.029

Abstract: Two groups of benzenesulfonamide derivatives, bearing pyrimidine moieties, were designed and synthesized as inhibitors of carbonic anhydrases (CA). Their binding affinities to six recombinant human CA isoforms I, II, VI, VII, XII, and XIII were determined by the thermal shift assay (TSA). The binding of several inhibitors was measured by isothermal titration calorimetry (ITC). Direct demonstration of compound inhibition was achieved by determining the inhibition constant by stopped-flow CO2 hydration assay. The most potent compounds demonstrated selectivity towards isoform I and affinities of 0.5nM. The crystal structures of selected compounds in complex with CA II, XII, and XIII were determined to atomic resolution. Compounds described here were compared with previously published pyrimidinebenzenesulfonamides.(1) Systematic structure-activity analysis of 40 compound interactions with six isoforms yields clues for the design of compounds with greater affinities and selectivities towards target CA isoforms.

Note: © Elsevier Ltd. ; Post referee fulltext in progress; Embargo 12 months from publication

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Contributing Institute(s):

1. EMBL-User (EMBL-User)

Research Program(s):

1. PETRA Beamline P14 (POF2-54G14) (POF2-54G14)
2. DORIS Beamline K1.1 (POF2-54G13) (POF2-54G13)

Experiment(s):

1. PETRA Beamline P14 (PETRA III)
2. DORIS Beamline K1.1 (DORIS III)

Appears in the scientific report 2013

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Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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