Journal Article

PHPPUBDB-23573

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Microfluidic devices for X-ray studies on hydrated cells

Weinhausen, B.Extern* ; Köster, S. (Corresponding author)Extern* ; DESY

2013
RSC Cambridge

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Please use a persistent id in citations: doi:10.1039/c2lc41014a  doi:10.3204/PHPPUBDB-23573

Abstract: X-ray studies of biological cells in microfluidic devices provide a method to probe cellular structures or structural changes at the molecular level in a precisely controlled environment. However, the device design and the used materials must be compatible with X-ray scattering techniques as well as the cell culture in the devices. For this purpose, we develop new types of X-ray compatible microfluidic devices, which are based on a UV-curable adhesive as a moldable material, and thin Kapton films and silicon nitride membrane windows as a growth substrate for cells and as a window material for X-rays. Using these devices, we perform scanning X-ray diffraction experiments with a nano-focused beam on fixed cells in buffer solution. In principle, these microfluidic devices also allow for X-ray studies on living cells.

Keyword(s): Cell Line (MeSH) ; Humans (MeSH) ; Microfluidic Analytical Techniques: instrumentation (MeSH) ; Microfluidic Analytical Techniques: methods (MeSH) ; Microscopy, Fluorescence (MeSH) ; Silicon Compounds: chemistry (MeSH) ; X-Rays (MeSH) ; Silicon Compounds ; silicon nitride

Note: © Royal Society of Chemistry

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Contributing Institute(s):

1. Experiments with synchrotron radiation (HASYLAB(-2012))

Research Program(s):

1. PETRA Beamline P10 (POF2-54G14) (POF2-54G14)

Experiment(s):

1. PETRA Beamline P10 (PETRA III)

Appears in the scientific report 2013

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Database coverage:
; ; No Author Disambiguation

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