Essential role of the metal-ion in the IPM-assisted domain closure of 3-isopropylmalate dehydrogenase

Graczer, E.; Svergun, D. I.; Zavodszky, P.; Bodonyi, A.; Bacso, A.; Szimler, T.; Vas, M.; Konarev, P. V.

doi:10.1016/j.febslet.2011.09.013

Journal Article

Essential role of the metal-ion in the IPM-assisted domain closure of 3-isopropylmalate dehydrogenase

Graczer, E. ; Konarev, P. V. ; Szimler, T. ; Bacso, A. ; Bodonyi, A. ; Svergun, D. I. ; Zavodszky, P. ; Vas, M. ; DESY

2011
Elsevier Amsterdam [u.a.]

FEBS letters 585, 3297-3302 (2011) [10.1016/j.febslet.2011.09.013]

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Abstract: X-ray structures of 3-isopropylmalate dehydrogenase (IPMDH) do not provide sufficient information on the role of the metal-ion in the metal-IPM assisted domain closure. Here solution studies were carried out to test its importance. Small-angle X-ray scattering (SAXS) experiments with the Thermus thermophilus enzyme (complexes with single substrates) have revealed only a very marginal (0-5%) extent of domain closure in the absence of the metal-ion. Only the metal-IPM complex, but neither the metal-ion nor the free IPM itself, is efficient in stabilizing the native protein conformation as confirmed by denaturation experiments with Escherichia coli IPMDH and by studies of the characteristic fluorescence resonance energy transfer (FRET) signal (from Trp to bound NADH) with both IPMDHs. A possible atomic level explanation of the metal-effect is given.

Keyword(s): 3-Isopropylmalate Dehydrogenase: chemistry (MeSH) ; Bacterial Proteins: chemistry (MeSH) ; Crystallography, X-Ray (MeSH) ; Escherichia coli: enzymology (MeSH) ; Metals: chemistry (MeSH) ; Protein Structure, Tertiary (MeSH) ; Thermus thermophilus: enzymology (MeSH) ; Bacterial Proteins ; Metals ; 3-Isopropylmalate Dehydrogenase