Journal Article

PHPPUBDB-20240

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519.

Bezsudnova, E. Y. ; Kovalchuk, M. V. ; Mardanov, A. V. ; Poliakov, K. M. ; Popov, V. O. ; Ravin, N. V. ; Skryabin, K. G. ; Smagin, V. A. ; Stekhanova, T. N. ; Tikhonova, T. V. ; DESY

2009
Blackwell Oxford [u.a.]

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Please use a persistent id in citations: doi:10.1107/S1744309109007799

Abstract: DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion method employing 35%(v/v) Tacsimate pH 7.0 as a precipitant and diffracted X-rays to 3.09 A resolution. They belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 79.7, c = 182.6 A.

Keyword(s): Crystallization (MeSH) ; Crystallography, X-Ray (MeSH) ; DNA Ligases: chemistry (MeSH) ; DNA Ligases: isolation & purification (MeSH) ; Electrophoresis, Polyacrylamide Gel (MeSH) ; Enzyme Stability (MeSH) ; Recombinant Proteins: chemistry (MeSH) ; Recombinant Proteins: isolation & purification (MeSH) ; Temperature (MeSH) ; Thermococcus: enzymology (MeSH) ; Recombinant Proteins ; DNA Ligases

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Contributing Institute(s):

1. European Molecular Biology Laboratory (EMBL)

Research Program(s):

1. DORIS Beamline K1.1 (POF1-550) (POF1-550)

Experiment(s):

1. DORIS Beamline K1.1 (DORIS III)

Appears in the scientific report 2009

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Database coverage:
; JCR ; No Author Disambiguation ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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