Journal Article

PHPPUBDB-19273

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structural basis of binding of fluorescent, site-specific dansylated amino acids to human serum albumin.

Ryan, A. J. ; Zunszain, P. A. ; Ghuman, J. ; Chung, C. W. ; Curry, S. ; DESY

2011
Elsevier San Diego, Calif.

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Please use a persistent id in citations: doi:10.1016/j.jsb.2010.10.004

Abstract: Human serum albumin (HSA) has two primary binding sites for drug molecules. These sites selectively bind different dansylated amino acid compounds, which-due to their intrinsic fluorescence-have long been used as specific markers for the drug pockets on HSA. We present here the co-crystal structures of HSA in complex with six dansylated amino acids that are specific for either drug site 1 (dansyl-l-asparagine, dansyl-l-arginine, dansyl-l-glutamate) or drug site 2 (dansyl-l-norvaline, dansyl-l-phenylalanine, dansyl-l-sarcosine). Our results explain the structural basis of the site-specificity of different dansylated amino acids. They also show that fatty acid binding has only a modest effect on binding of dansylated amino acids to drug site 1 and identify the location of secondary binding sites.

Keyword(s): Arginine: analogs & derivatives (MeSH) ; Arginine: metabolism (MeSH) ; Asparagine: analogs & derivatives (MeSH) ; Asparagine: metabolism (MeSH) ; Dansyl Compounds: metabolism (MeSH) ; Glutamates: metabolism (MeSH) ; Humans (MeSH) ; Phenylalanine: metabolism (MeSH) ; Protein Binding (MeSH) ; Sarcosine: analogs & derivatives (MeSH) ; Sarcosine: metabolism (MeSH) ; Serum Albumin: metabolism (MeSH) ; Dansyl Compounds ; Glutamates ; Serum Albumin ; dansyl-phenylalanine ; Sarcosine ; dansylsarcosine ; dansyl asparagine ; dansyl-L-arginine ; Phenylalanine ; dansylglutamic acid ; Asparagine ; Arginine

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Contributing Institute(s):

1. European Molecular Biology Laboratory (EMBL)

Research Program(s):

1. DORIS Beamline K1.3 (POF2-54G13) (POF2-54G13)
2. DORIS Beamline K1.1 (POF2-54G13) (POF2-54G13)

Experiment(s):

1. DORIS Beamline K1.1 (DORIS III)
2. DORIS Beamline K1.3 (DORIS III)

Appears in the scientific report 2011

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Database coverage:
; JCR ; No Author Disambiguation ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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